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Biofilms

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Biofilms are complex communities of microorganisms encased in a self-produced extracellular polysaccharide matrix attached to surfaces. These microbial consortia can include single or multiple species, providing enhanced survival benefits by forming organized, multilayered structures.The formation of biofilms occurs through four key stages: attachment, colonization, development, and dispersal.During attachment, free-swimming planktonic cells adhere to a surface, often facilitated by...

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Updated: Mar 6, 2026

Synthesis of Hydrogels with Antifouling Properties As Membranes for Water Purification

Synthesis of Hydrogels with Antifouling Properties As Membranes for Water Purification

Published on: April 7, 2017

Synthetic Biofilms for Green Membranes: Engineering Low-Energy Filtration Systems.

Chenyang Zhou^1,2, Tingfeng Cheng³, Jinjie Zhou^1,2

¹School of Environmental Science and Engineering, Tianjin University, Tianjin 300350, PR China.

Environmental Science & Technology

|March 5, 2026

Summary

This summary is machine-generated.

Engineered bacteria control membrane biofouling using quorum-quenching (QQ) technology. This sustainable approach reduces energy use and environmental impact in water purification.

Keywords:

CRISPR-Cas9 biofilm formation inhibition gene expression regulation microbial gene editing quorum quenching

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Three-Dimensionally Printed Microfluidic Cross-flow System for Ultrafiltration/Nanofiltration Membrane Performance Testing

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Last Updated: Mar 6, 2026

Synthesis of Hydrogels with Antifouling Properties As Membranes for Water Purification

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Published on: April 7, 2017

Proof-of-Concept for Gas-Entrapping Membranes Derived from Water-Loving SiO2/Si/SiO2 Wafers for Green Desalination

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Three-Dimensionally Printed Microfluidic Cross-flow System for Ultrafiltration/Nanofiltration Membrane Performance Testing

Published on: February 13, 2016

Area of Science:

Environmental Engineering
Biotechnology
Materials Science

Background:

Membrane filtration is vital for water purification but faces sustainability challenges due to biofouling.
Biofouling increases energy consumption and environmental impact, while conventional control methods have limitations.

Purpose of the Study:

To develop an engineered bacterium for inducible control of biofilm structure in membrane filtration.
To assess the efficacy of this approach in mitigating biofouling and improving membrane performance.

Main Methods:

Constructed an inducible, engineered quorum-quenching (QQ) bacterium using genomic integration and CRISPR-Cas gene editing.
Expressed the 'aiiO' gene under l-ribose induction for biofilm regulation.
Validated the system using a gravity-driven membrane ultrafiltration system (UF-GDM) model.

Main Results:

Engineered bacteria effectively reduced extracellular polymeric substances (EPS), increased biofilm porosity, and decreased stickiness.
Achieved a 64.5% reduction in transmembrane pressure, a 13.2% increase in TOC removal, and a 16.1% extension in membrane lifespan.
Demonstrated significant economic benefits, including a 31.52% annual net profit increase and a 27.43% reduction in carbon footprint for a large-scale plant.

Conclusions:

This novel QQ bacterium strategy offers effective biofouling resistance for membrane filtration.
The approach promotes sustainable, low-energy water purification, enhancing technology adoption.
Provides a viable solution for improving the efficiency and environmental profile of membrane-based water treatment.