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UV–Vis Spectrometers01:14

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The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell.
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Portable colorimetric absorbance reader with extended dynamic range via time-resolved sensing.

S Moreno1, O Caravaca-Müller2, X García-Fernández3

  • 1Department of Electronic and Biomedical Engineering, Faculty of Physics, Universitat de Barcelona (UB), Barcelona, Spain; Institute for Nanoscience and Nanotechnology (IN2UB), Universitat de Barcelona (UB), 08028, Barcelona, Spain.

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|March 5, 2026
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Summary
This summary is machine-generated.

This study introduces a portable reader using single-photon avalanche diode (SPAD) arrays for time-resolved photon counting. This advanced absorbance reader extends dynamic range and improves quantification for bioanalytical methods, enhancing point-of-care diagnostics.

Keywords:
Convolutional neural network (CNN)High dynamic rangeMagneto-immunoassayPortable absorbance readerSingle-photon avalanche diode (SPAD)Time-resolved sensing

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Area of Science:

  • Bioanalytical Chemistry
  • Photonics
  • Medical Diagnostics

Background:

  • Colorimetric bioanalytical methods often saturate at high analyte concentrations, necessitating serial dilutions.
  • Conventional absorbance readers have limitations in dynamic range for samples with varying analyte levels.

Purpose of the Study:

  • To develop a portable absorbance reader with an extended dynamic range using time-resolved photon counting.
  • To overcome the saturation limitations of conventional readers in bioanalytical assays.

Main Methods:

  • Utilized a single-photon avalanche diode (SPAD) array for in-pixel time-resolved photon counting.
  • Employed a lifetime-based measurement principle to derive absorbance from photon-arrival decay constants (τ).
  • Applied the reader to an influenza B nucleoprotein magnetic particle immunoassay.

Main Results:

  • The SPAD-based reader demonstrated a monotonic response up to 64 ng/mL, significantly exceeding the 12.5 ng/mL saturation of a commercial photometer.
  • Achieved low limits of detection (0.0034 ng/mL) and quantification (0.0949 ng/mL) with coefficients of variation <10%.
  • A convolutional neural network accurately inferred analyte concentration (R² = 0.986) from time-resolved photon data.

Conclusions:

  • The SPAD-based reader extends the usable absorbance range and maintains sensitivity, outperforming commercial equipment.
  • The time-resolved photon counting approach allows for both physical modeling and data-driven inference for accurate quantification.
  • This technology offers a pathway for high-performance, portable point-of-care diagnostic solutions.