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Optimization of Synthetic Proteins: Identification of Interpositional Dependencies Indicating Structurally and/or Functionally Linked Residues
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Conformational selection and linker-dependent specificity in the MAGI-1 WW tandem.

Julian Toso1, Eduarda Santos Ventura1, Valeria Pennacchietti1

  • 1Dipartimento di Scienze Biochimiche "A. Rossi Fanelli", Sapienza Università di Roma - Laboratory affiliated to Istituto Pasteur Italy - Fondazione Cenci Bolognetti, 00185, Rome, Italy.

International Journal of Biological Macromolecules
|March 5, 2026
PubMed
Summary

The MAGI-1 WW tandem scaffold enhances protein binding affinity and specificity by stabilizing complexes and utilizing conformational selection. Its structure, sensitive to linker length and pH, enables precise regulation of protein interactions.

Keywords:
BindingConformational selectionKineticsTandem domains

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