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Protein Dynamics in Living Cells

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

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Related Experiment Video

Updated: Mar 7, 2026

Separation of Spinach Thylakoid Protein Complexes by Native Green Gel Electrophoresis and Band Characterization using Time-Correlated Single Photon Counting

Separation of Spinach Thylakoid Protein Complexes by Native Green Gel Electrophoresis and Band Characterization using Time-Correlated Single Photon Counting

Published on: February 14, 2019

State transitions in Physcomitrium patens studied with time-resolved fluorescence

Dana Verhoeven¹, Cleo Bagchus¹, Lotte Jore¹

¹Laboratory of Biophysics, Wageningen University, Wageningen, 6708 WE, The Netherlands.

Photosynthesis Research

|March 5, 2026

Summary

No abstract available in PubMed .

Keywords:

Physcomitrium patens Light harvesting Photosynthesis Photosystem I State transitions Time-resolved fluorescence

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