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Published on: May 10, 2022
The Correlation Between NTCP rs2296651 Variant and Risk of Hepatitis B, Cirrhosis, and Hepatocellular Carcinoma in
Hassan Akrami1, Mohammad Reza Fattahi1, Mastaneh Zeraatiannejad1
1Gastroenterohepatology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Insights
The NTCP rs2296651 variant shows a protective effect against Hepatitis B virus (HBV) infection in the Iranian population. This single nucleotide polymorphism (SNP) may influence the development of liver cirrhosis and hepatocellular carcinoma (HCC).
Area of Science:
- Hepatology and viral hepatitis research.
- Genetic variations and disease susceptibility.
- Molecular mechanisms of viral entry.
Background:
- Hepatitis B virus (HBV) infection is a major global health concern, leading to liver cirrhosis and hepatocellular carcinoma (HCC).
- HBV entry into hepatocytes is mediated by the sodium taurocholate co-transporting polypeptide (NTCP).
- The NTCP rs2296651 single nucleotide polymorphism (SNP) (Ser267Phe) has been linked to altered NTCP function and HBV entry in Asian populations.
Purpose of the Study:
- To investigate the association between the NTCP rs2296651 variant and HBV infection, liver cirrhosis, and HCC in the Iranian population.
- To determine if this genetic variation influences susceptibility to HBV-related liver diseases.
Main Methods:
- DNA was extracted from 50 healthy controls and 90 patients with HBV, cirrhosis, or HCC.
- Genotyping for the NTCP rs2296651 variant was performed using tetra-amplification refractory mutation system polymerase chain reaction (tetra-ARMS PCR).
- Statistical analysis was conducted using SPSS software.
Main Results:
- A significant positive association was found between the NTCP rs2296651 variant and cirrhosis in the control group (P=0.002).
- A significant positive association was observed between cirrhosis and HBV infection (P<0.001).
- A significant negative relationship was identified between cirrhosis and HCC (P=0.003).
Conclusions:
- The NTCP rs2296651 variant may confer resistance to HBV infection in the Iranian population.
- This SNP might play a role in the pathogenesis of HBV-related liver diseases, including cirrhosis and HCC, in this demographic.
Background:
Millions of people are suffering from different types of liver diseases worldwide. Hepatitis B, cirrhosis, and hepatocellular carcinoma (HCC) are the leading causes of death caused by the hepatitis B virus (HBV). HBV needs to interact with the sodium taurocholate co-transporting polypeptide (NTCP) to enter the cytoplasm of the hepatocyte. single nucleotide polymorphism (SNP) with rs2296651 (Ser267Phe, S267F, G>A) variation in the NTCP has been investigated as a reverse association with the function of NTCP and entry of HBV into the cell in the Asian population. We investigated the relationship between the NTCP rs2296651 variant and HBV infection, liver cirrhosis, and HCC in the Iranian population.
Methods:
Whole blood DNA of 50 healthy individuals as a control group and 90 patients (HBV, cirrhosis, and HCC) were extracted, and the tetra amplification refractory mutation system polymerase chain reaction (tetra-ARMS PCR) was done to identify the genotypes of the samples.
Results:
Based on our analytical tests using SPSS software, there was a positive and significant association between NTCP rs2296651 in the control group and cirrhosis (P=0.002), as well as between cirrhosis and HBV (P<0.001). However, there was a negative relationship between cirrhosis and HCC (P=0.003).
Conclusion:
The NTCP rs2296651 variant may confer resistance to HBV infection in the Iranian population.
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