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Cotton boll extraction and single-boll weight estimation based on UAV multispectral imagery
Maoguang Chen1, Caixia Yin1, Na Su1
1Engineering Research Centre of Cotton, Ministry of Education/College of Agriculture, Xinjiang Agricultural University, Urumqi, China.
Frontiers in Plant Science
|March 6, 2026
Summary
A new UAV multispectral workflow accurately estimates single-boll weight (SBW) in cotton by combining object-based boll extraction and machine learning. This method overcomes background interference for improved crop management and breeding evaluations.
Area of Science:
- Agricultural Science
- Remote Sensing
- Machine Learning
Background:
- Estimating single-boll weight (SBW) in cotton is challenging post-defoliation due to spectral interference from lint, soil, and senescent leaves.
- Existing methods struggle with background noise, limiting accurate spatial estimation of SBW.
Purpose of the Study:
- To develop and validate a Unmanned Aerial Vehicle (UAV) multispectral workflow for improved single-boll weight (SBW) mapping in cotton.
- To reduce background interference and enhance the transferability of SBW estimation models.
Main Methods:
- A workflow integrating object-based boll extraction (using maximum likelihood classification), spectral feature selection (Pearson correlation and SHAP), and machine learning regression (ridge, random forest, neural network) was employed.
- Data were collected from a two-year cotton experiment with varying varieties and planting densities.
- Maximum likelihood classification achieved >97% accuracy for boll extraction.
Main Results:
- The optimal model, combining maximum likelihood boll extraction with neural network regression, achieved a coefficient of determination of 0.80 and RMSE of 0.31 g.
- Vegetation indices from red, red-edge, and near-infrared bands, especially those mitigating soil effects, showed strong SBW correlation.
- Relative errors were consistently below 15% across different experimental conditions.
Conclusions:
- The proposed UAV multispectral workflow effectively reduces background interference for accurate SBW spatial estimation.
- This approach enables transferable SBW mapping crucial for cotton breeding programs, density optimization, and harvest management.

