Establishment of an industrialized micropropagation system for Cerasus campanulata Maxim

Cerasus campanulata Maxim. possesses high ornamental value owing to its early flowering period, holding considerable application potential. However, research on its propagation techniques is still in the preliminary stage. To establish an efficient regeneration system for C. campanulata, this study used tissue-cultured seedlings as explants and employed an orthogonal experimental design to investigate the effects of key factors at different culture stages on plant regeneration. The results indicated that the suitable proliferation medium was ½MS supplemented with 0.1 mg/L NAA, 0.5 mg/L 6-BA, and 2.5 g/L banana powder. With this medium, the survival rate reached 100%, the proliferation rate was 96.67%, the proliferation coefficient exceeded 4, and the average bud height was 4.57 cm. Furthermore, the regenerated buds exhibited vigorous and healthy growth. The suitable rooting medium was ½MS, which yielded a rooting rate of over 88% and a survival rate of over 88% after greenhouse acclimatization. After 120 days of pot cultivation, the regenerated plants achieved an average plant height of 57.83 cm, an average leaf length of 15.03 cm, an average leaf width of 6.31 cm, and an average of 17 leaves per plant. Genetic stability analysis of the regenerated plantlets after three subculture cycles showed no obvious genetic variation. In conclusion, this study successfully established a practical regeneration system for C. campanulata, covering proliferation, rooting, acclimatization, pot cultivation, and preliminary genetic stability verification, which provides a reliable technical basis for the large-scale propagation and application of this species.