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Immunogold Electron Microscopy

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.

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Related Experiment Video

Updated: Mar 7, 2026

Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays

Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays

Published on: June 23, 2022

Magneto-Plasmonics-Enhanced Colorimetric Lateral Flow Immunoassay Using Magnetic-Gold Nanostars.

Supriya Atta^1,2, Taylor L Thorsen^1,3, Yuanhao Zhao^1,2

¹Fitzpatrick Institute for Photonics, Duke University, Durham, North Carolina 27708, United States.

ACS Applied Materials & Interfaces

|March 6, 2026

Summary

A new magneto-plasmonics enhanced colorimetric lateral flow immunoassay (mpLFIA) significantly boosts infectious disease detection sensitivity. This novel platform uses magnetic gold nanostars for a 1000-fold improvement in detecting viruses like Rift Valley fever virus (RVFV).

Keywords:

LFIA colorimetric magnetic nanostar sensing

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Synthesis of Immunotargeted Magneto-plasmonic Nanoclusters

Synthesis of Immunotargeted Magneto-plasmonic Nanoclusters

Published on: August 22, 2014

Area of Science:

Biomedical Engineering
Nanotechnology
Analytical Chemistry

Background:

Colorimetric lateral flow immunoassays (LFIA) offer rapid, accessible point-of-care testing (POCT) for infectious diseases.
Conventional LFIA sensitivity is limited by weak signal intensity from gold nanospheres (GNPs).

Purpose of the Study:

To develop a highly sensitive LFIA platform overcoming the limitations of conventional assays.
To enhance diagnostic potential for infectious diseases through improved detection limits.

Main Methods:

Development of a magneto-plasmonics enhanced colorimetric LFIA (mpLFIA) using a hybrid nanoparticle system: magnetic gold nanostars (mpGNS).
Utilizing dual enhancement mechanisms: magnetic preconcentration and plasmonic amplification.
Evaluation of mpGNS variants using Rift Valley fever virus (RVFV) nucleoprotein as a model analyte.

Main Results:

The mpLFIA platform achieved an outstanding limit of detection (LOD) of 2.24 pg/mL for RVFV nucleoprotein.
Demonstrated a 1000-fold sensitivity enhancement compared to conventional GNP-based LFIA.
Identified mpGNS-3, with longest spikes and highest branch density, as the optimal signal amplifier.

Conclusions:

The developed mpLFIA platform offers unprecedented signal intensity and detection performance.
This technology holds significant potential as a sensitive and portable bioanalytical tool for POCT.
The mpLFIA represents a breakthrough for improving infectious disease diagnostics at the point-of-care.