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Updated: Mar 7, 2026

Enrichment of Bacterial Lipoproteins and Preparation of N-terminal Lipopeptides for Structural Determination by Mass Spectrometry
Published on: May 21, 2018
The Calcium-Dependent Cyclic Lipopeptide Antibiotic Superfamily-Structural Diversity and Biological Activity
Jeremy Goodyear1, Scott D Taylor1
1Dept. of Chemistry, University of Waterloo, 200 University Ave. West, Waterloo, Ontario N2L 3G1, Canada.
None:
The rapid emergence of multidrug-resistant (MDR) bacteria threatens the effectiveness of existing antibiotics and has created an urgent need for agents with novel mechanisms of action. Calcium-dependent antibiotics (CDAs), a structurally diverse group of nonribosomal cyclic lipopeptides, have gained attention for their potent activity against Gram-positive pathogens, including strains resistant to conventional therapies. Their hallmark feature is Ca2+-mediated activation: the calcium ion binding to the lipopeptide enables selective interactions with bacterial lipids or cell-wall precursors. This review examines the relationship between CDA structure, Ca2+ coordination, mechanistic diversity, and biological activity. Together, these features position CDAs as promising templates for next-generation therapeutics to combat the escalating MDR crisis.
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