Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

96-Well Agarose-Gel Electromembrane Extraction.

Analytical chemistry·2025
Same author

Automated Formation of Supported Liquid Membranes by Molecular Self-Assembly: A Step Forward for Liquid-Phase Microextraction.

Analytical chemistry·2025
Same author

Development of a Thiol-ene Microfluidic Chip for Hydrogen/Deuterium Exchange Mass Spectrometry (HDX-MS).

Analytical chemistry·2025
Same author

Electromembrane extraction of peptides based on charge, hydrophobicity, and size - A large-scale fundamental study of the extraction window.

Journal of separation science·2024
Same author

Microfluidic isolation of extrachromosomal circular DNA through selective digestion of plasmids and linear DNA using immobilized nucleases.

Lab on a chip·2024
Same author

Optimization of generic conditions for electromembrane extraction of basic substances of moderate or low polarity.

Journal of separation science·2024

Related Experiment Video

Updated: Mar 7, 2026

Author Spotlight: Asymmetric Field Flow Fractionation for Bioreactor Integration
06:28

Author Spotlight: Asymmetric Field Flow Fractionation for Bioreactor Integration

Published on: February 2, 2024

1.5K

Microfluidic device for electromembrane extraction with a micro-pillar stabilized liquid membrane.

Anna Thu Hoai Nguyen1, Nickolaj J Petersen1, Stig Pedersen-Bjergaard1,2

  • 1Department of Pharmacy, Faculty of Health and Medical Sciences, University of Copenhagen, Universitetsparken 2, Copenhagen, 2100, Denmark.

Analytical and Bioanalytical Chemistry
|March 6, 2026
PubMed
Summary

A novel microfluidic device for electromembrane extraction (EME) utilizes micro-pillars to stabilize the liquid membrane. This innovative design enables rapid membrane replacement, low-voltage operation, and reusability, enhancing sample cleanup efficiency.

Keywords:
Electromembrane extractionMicroextractionMicrofluidic deviceSample preparationStabilized interfaces

More Related Videos

Merging Ion Concentration Polarization between Juxtaposed Ion Exchange Membranes to Block the Propagation of the Polarization Zone
08:06

Merging Ion Concentration Polarization between Juxtaposed Ion Exchange Membranes to Block the Propagation of the Polarization Zone

Published on: February 23, 2017

9.0K
Pneumatically Driven Microfluidic Platform for Micro-Particle Concentration
08:43

Pneumatically Driven Microfluidic Platform for Micro-Particle Concentration

Published on: February 1, 2022

2.9K

Related Experiment Videos

Last Updated: Mar 7, 2026

Author Spotlight: Asymmetric Field Flow Fractionation for Bioreactor Integration
06:28

Author Spotlight: Asymmetric Field Flow Fractionation for Bioreactor Integration

Published on: February 2, 2024

1.5K
Merging Ion Concentration Polarization between Juxtaposed Ion Exchange Membranes to Block the Propagation of the Polarization Zone
08:06

Merging Ion Concentration Polarization between Juxtaposed Ion Exchange Membranes to Block the Propagation of the Polarization Zone

Published on: February 23, 2017

9.0K
Pneumatically Driven Microfluidic Platform for Micro-Particle Concentration
08:43

Pneumatically Driven Microfluidic Platform for Micro-Particle Concentration

Published on: February 1, 2022

2.9K

Area of Science:

  • Analytical Chemistry
  • Separation Science
  • Microfluidics

Background:

  • Electromembrane extraction (EME) is a selective sample cleanup technique.
  • Conventional EME formats face limitations in membrane replacement, voltage requirements, and reusability.

Purpose of the Study:

  • To introduce a microfluidic device for EME with a micro-pillar-stabilized liquid membrane.
  • To address limitations of conventional EME by enabling rapid membrane replacement, low-voltage operation, and extended reuse.

Main Methods:

  • Fabrication of a microchip using a stoichiometric thiol-ene polymer.
  • Optimization of extraction parameters including flow rates, liquid membrane volume, and extraction time.
  • Lidocaine was used as the model analyte.

Main Results:

  • Recoveries up to 50% were achieved with optimized parameters (0.5 µL min-1 sample flow, 2.0 µL min-1 acceptor flow, 0.1 µL NPOE membrane, 10 min extraction).
  • Excellent linearity (R2≈0.99 from 5-20 µg mL-1), acceptable repeatability (16.0% RSD), and minimal carryover (1.3%) were demonstrated.
  • The system showed stability for 60 min continuous operation, with easy membrane replenishment and a high AGREEprep greenness score (0.79).

Conclusions:

  • The developed microfluidic EME device offers a versatile, reusable, and sustainable platform.
  • The design overcomes key limitations of traditional EME, paving the way for flexible on-site analytical applications.
  • This work represents a significant advancement in microextraction technology.