Related Experiment Video

Updated: May 2, 2026

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes
07:59

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes

Published on: March 25, 2014

15.7K

Computational prediction and molecular simulation-based development of a multi-epitope mRNA vaccine targeting capsule

Shubhi Singh1, Priya Swaminathan1

  • 1Department of Biotechnology, School of Bioengineering, College of Engineering and Technology, SRM Institute of Science and Technology, Kattankulathur, Chengalpattu, Tamil Nadu 603203, India.

Computational Biology and Chemistry
|March 6, 2026
PubMed

Insights

Developing an Enterobacter cloacae (EC) vaccine is crucial. This study identified 12 epitopes for an mRNA vaccine, showing promise in silico for preventing EC infections.

Area of Science:

  • Microbiology
  • Vaccinology
  • Bioinformatics

Background:

  • Enterobacter cloacae (EC) is a significant opportunistic pathogen causing diverse infections.
  • The emergence of EC necessitates the development of novel preventive strategies, such as vaccines.

Purpose of the Study:

  • To identify potential epitopes from Enterobacter cloacae capsule polysaccharide (CPS) proteins for mRNA vaccine development.
  • To design and evaluate an in silico mRNA vaccine candidate against EC infections.

Main Methods:

  • Utilized immunoinformatic approaches to identify T-cell and B-cell epitopes.
  • Employed molecular docking and dynamics simulations to predict epitope-MHC interactions and complex stability.
  • Performed in silico immunological modeling and codon optimization for mRNA vaccine design.

Main Results:

  • Identified 12 T-helper (HTL), cytotoxic T-lymphocyte (CTL), and linear B-cell (LBL) epitopes.
  • Successfully designed three distinct vaccine constructs with adjuvants.
  • In silico analysis indicated vaccine stability and potential efficacy, with successful docking to Toll-Like Receptors 2 and 4.

Conclusions:

  • The proposed in silico mRNA vaccine construct is a promising candidate for combating Enterobacter cloacae infections.
  • Further in vitro studies are warranted to validate the efficacy of this novel vaccine approach.
Keywords:
Capsule polysaccharide-protein (CPS)HumoralImmunoinformaticsInsilicoMolecular dynamics

More Related Videos

Production of E. coli-expressed Self-Assembling Protein Nanoparticles for Vaccines Requiring Trimeric Epitope Presentation10:58

Production of E. coli-expressed Self-Assembling Protein Nanoparticles for Vaccines Requiring Trimeric Epitope Presentation

Published on: August 21, 2019

8.1K
A "Plug-And-Display" Nanoparticle Vaccine Platform Based on Outer Membrane Vesicles Displaying SARS-CoV-2 Receptor-Binding Domain08:07

A "Plug-And-Display" Nanoparticle Vaccine Platform Based on Outer Membrane Vesicles Displaying SARS-CoV-2 Receptor-Binding Domain

Published on: July 25, 2022

3.1K

Related Concept Videos

Biosynthesis in Bacteria01:24

Biosynthesis in Bacteria

Biosynthesis in bacteria is a fundamental anabolic process that generates essential macromolecules, including proteins, nucleic acids, lipids, and polysaccharides. These macromolecules are critical for cellular growth, replication, and function. The process is tightly regulated and energetically linked to catabolic pathways to ensure optimal resource utilization.Biosynthetic pathways begin with precursor metabolites such as pyruvate, acetyl-CoA, and glucose-6-phosphate derived from glycolysis,...
1.0K
Biosynthesis of Polysaccharides01:26

Biosynthesis of Polysaccharides

Polysaccharides such as glycogen and starch are synthesized from nucleoside diphosphate sugars, primarily uridine diphosphate glucose (UDPG) and adenosine diphosphate glucose (ADPG). These activated glucose donors act as key intermediates in carbohydrate metabolism and biosynthesis. UDPG primarily involves glycogen synthesis in animals and many bacteria, while ADPG plays a fundamental role in starch synthesis in plants and certain bacteria.UDPG is formed when glucose-1-phosphate reacts with...
984
Amino Acid Biosynthetic Pathways01:29

Amino Acid Biosynthetic Pathways

Amino acid biosynthesis is essential for cell growth, protein synthesis, and metabolic regulation. Cells generate essential and non-essential amino acids from metabolic intermediates to sustain vital biological functions. These intermediates originate from key metabolic pathways: glycolysis, the tricarboxylic acid (TCA) cycle, and the pentose phosphate pathway. Important precursors include α-ketoglutarate, pyruvate, oxaloacetate, phosphoenolpyruvate, and erythrose-4-phosphate, which...
1.8K
Bioreactor Controls-III01:22

Bioreactor Controls-III

Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...
67
Production of Antibiotics01:27

Production of Antibiotics

Penicillin, one of the earliest and most widely used antibiotics, is produced industrially by the filamentous fungus Penicillium chrysogenum. Large stirred-tank bioreactors ranging from tens to hundreds of thousands of liters maintain tightly controlled temperature, pH, and dissolved oxygen conditions to support fungal metabolism and maximize antibiotic yield. Penicillin is a secondary metabolite, synthesized primarily during the stationary growth phase, which requires a carefully managed...
265