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Engineering microbial cell factories for succinic acid biomanufacturing: Challenges and perspectives
Ling-Ling Bai1, Na Li1, Jing-Ru Yang1
1State Key Laboratory of Synthetic Biology, Tianjin University, Tianjin, China; Frontiers Science Center for Synthetic Biology (Ministry of Education), Tianjin University, Tianjin, China; School of Synthetic Biology and Biomanufacturing, Tianjin University, Tianjin, China.
Biotechnology Advances
|March 6, 2026
Summary
Microbial biosynthesis offers a sustainable route to bio-based succinic acid production using renewable resources. This review evaluates microbial platforms and advanced strategies for efficient industrial-scale production.
Area of Science:
- Biotechnology
- Industrial Microbiology
- Chemical Engineering
Background:
- Succinic acid is a vital platform chemical with sustainable production potential from renewable feedstocks.
- Microbial biosynthesis presents a viable alternative to traditional petrochemical methods for succinic acid generation.
- Developing efficient bio-based production is crucial for a circular economy.
Purpose of the Study:
- To systematically review and compare microbial platforms for succinic acid biosynthesis.
- To explore rational pathway design, synthetic biology tools, and downstream processing innovations.
- To provide a framework for optimizing industrial-scale bio-based succinic acid production.
Main Methods:
- Comparative analysis of bacterial and yeast microbial platforms.
- Evaluation of metabolic engineering strategies for pathway optimization.
- Review of synthetic biology tools and downstream separation techniques.
- Integration of systems biology, metabolic modeling, and process engineering.
Main Results:
- Identification of key physiological traits and metabolic capabilities of microbial platforms.
- Assessment of rational design principles for biosynthesis pathways.
- Highlighting advancements in synthetic biology for enhanced chassis performance.
- Discussion of innovations in downstream separation for improved economics.
Conclusions:
- Multidisciplinary approaches are essential for overcoming challenges in carbon flux, redox balancing, and scaling.
- Efficient microbial cell factories are key to accelerating commercialization of bio-based succinic acid.
- This review offers a strategic framework for advancing sustainable succinic acid production.
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