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Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput
Published on: March 30, 2018
Arabidopsis acclimation to daily environmental fluctuations converts a defense response regulator into a
Marie Didelon1, Justine Sucher1, Florent Delplace1
1Laboratoire des Interactions Plantes-Microbes-Environnement (LIPME), Université de Toulouse, INRAE, CNRS, F-31326, Castanet-Tolosan, France.
Abstract:
Acclimation enables plants to adjust to immediate environmental fluctuations and is therefore key to the resilience of plant disease resistance in a time of climate change. Here, we report on the acclimation of Arabidopsis thaliana quantitative immune responses against the fungal pathogen Sclerotinia sclerotiorum to daily environmental fluctuations. We analyzed disease resistance phenotypes and global gene expression in plants grown in three acclimation regimes, revealing the rewiring of regulatory networks during this process. We identified pathogen-induced genes weakly sensitive to acclimation as promising bases for acclimation-proof immunity. Fluctuations in Mediterranean-like acclimation resulted in an increased disease susceptibility and the misregulation of many pathogen-responsive genes. We identified A. thaliana mutants in novel immune components contributing positively to quantitative disease resistance following temperate but not Mediterranean acclimation. Quantitative disease resistance was maintained under Mediterranean acclimation in NAC42-like mutants and associated with a switch in the repertoire of pathogen-responsive targets of this transcription factor. Our work reveals the role of immune gene networks' plasticity in acclimation and suggests new strategies to maintain plant immune function in a warming climate.
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Transcription
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
Responses to Heat and Cold Stress
Cell Signaling in Plants
Responses to Drought and Flooding
Responses to Salt Stress