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The Importance of Correct Protein Concentration for Kinetics and Affinity Determination in Structure-function Analysis
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Quantitative analysis of ternary complex kinetics by a surface immobilization method

Xiaohan Mai1, Yang Luo2, Ruoxin Fang3

  • 1Shanghai Engineering Research Center of Ultra-precision Optical Manufacturing, Key Laboratory of Micro and Nano Photonic Structures (Ministry of Education), College of Future Information Technology, Fudan University, Shanghai, 200433, China; Quzhou Fudan Institute, 108 Minjiang Avenue, Kecheng District, Quzhou, Zhejiang Province, China.

Biochemical and Biophysical Research Communications
|March 7, 2026
PubMed

Keywords:
Cooperativity factorHook effectOblique-incidence reflectivity differenceTarget protein degradationTernary complex kinetics

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