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Illuminating epilepsy: A deep NIR-I fluorogenic sensor reports peroxynitrite dynamics in vivo.

Ruiqi Han1, Lingli Wang1, Xiaoteng Ma1

  • 1Key Laboratory for Special Functional Materials of Ministry of Education, School of Nanoscience and Materials Engineering, Henan University, Kaifeng 475004, PR China.

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PubMed
Summary

A new deep NIR-I fluorogenic probe, BDP-NIR-S-Fa, effectively visualizes peroxynitrite (ONOO-) in epilepsy models. This probe aids in diagnosing epilepsy and evaluating antiepileptic drug efficacy.

Keywords:
BODIPYBioimagingDeep NIR I fluorescent probeDiagnosis of epilepsyPeroxynitrite

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Area of Science:

  • Biomedical Engineering
  • Chemical Biology
  • Neuroscience

Background:

  • Peroxynitrite (ONOO-) is implicated in epilepsy pathogenesis.
  • Real-time monitoring of ONOO- dynamics is crucial for understanding epilepsy and developing therapies.

Purpose of the Study:

  • To develop and validate a novel deep NIR-I fluorogenic probe (BDP-NIR-S-Fa) for sensitive and specific detection of ONOO-.
  • To assess the probe's utility in visualizing ONOO- in epilepsy models and evaluating therapeutic responses.

Main Methods:

  • Synthesis and characterization of the BDP-NIR-S-Fa probe.
  • In vitro and in vivo imaging of ONOO- in kainic acid-induced epilepsy models.
  • Evaluation of antiepileptic drug efficacy by monitoring ONOO- reduction.
  • Testing the probe in a lipopolysaccharide (LPS)-induced peritonitis model.

Main Results:

  • BDP-NIR-S-Fa exhibits specific emission at 805 nm and rapid, sensitive response to ONOO-.
  • The probe successfully visualized elevated ONOO- in epileptic cells and a mouse model.
  • BDP-NIR-S-Fa effectively monitored ONOO- reduction following antiepileptic drug treatment.
  • The probe demonstrated utility for in vivo ONOO- imaging in an LPS-induced peritonitis model.

Conclusions:

  • BDP-NIR-S-Fa is a robust sensor for real-time tracking of ONOO- biology.
  • The probe offers a direct method to illuminate ONOO-'s role in seizure pathology.
  • This sensor advances early diagnosis and therapy assessment for epilepsy.