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Updated: Jul 17, 2026
Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria
Published on: May 8, 2013
A novel fluorescence method for sensitive detection of amikacin residues in milk samples: Greenness, blueness, and
Abobakr A Mohamed1, Shaima M Hamdy2, Hassan M A Abdelbaky2
1Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Fayoum University, Faiyum, Egypt.
Abstract:
Amikacin is an antibiotic commonly employed in veterinary medicine primarily for treatment and prevention of certain diseases. This study describes sensitive and simple spectrofluorimetric approach for the detection of amikacin in real milk samples that adopts a one pot-synthesis of a fully conjugated, planar, and cationic diarylpyrrolone derivative, this fluorogenic reaction allows sensitive determination of amikacin in milk at an emission wavelength of 485 nm after excitation at 390 nm, for the first time. The limits of detection and quantification were 20.60 and 62.50ngmL-1, respectively over a concentration range of 80.0-400.0, with good recovery. The facile application of the proposed method paves the way for its implementation in monitoring the potential risks posed by residual antibiotics to public health and ecological integrity.
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