Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Renewal of Intestinal Stem Cells01:23

Renewal of Intestinal Stem Cells

3.4K
The intestinal epithelial lining rapidly renews every 4 to 5 days. The renewal is facilitated by intestinal stem cells (ISCs) located at the base of the crypt– a gland located at the bottom of each villus. ISCs divide asymmetrically to form new stem cells and progenitor daughter cells. The daughter cells are called transit-amplifying (TA) cells which move upwards along the crypt and either differentiate into absorptive cells– the enterocytes or secretory cells– including the...
3.4K
Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

2.6K
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.6K
Adult Stem Cells01:33

Adult Stem Cells

34.0K
Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously...
34.0K
Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal01:22

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

2.8K
Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
2.8K
Mucosal Barrier of the Stomach01:25

Mucosal Barrier of the Stomach

2.6K
The gastric glands contain parietal cells that secrete hydrochloric acid (HCl) for digestion. The cells secrete HCl because it is highly corrosive and essential for breaking down food. To achieve this, they secrete hydrogen and chloride ions into the lumen of the gastric glands, which combine to form HCl.
Within parietal cells, carbonic acid is first formed through the reaction of water and carbon dioxide. The dissociation of carbonic acid releases bicarbonate and hydrogen ions. The bicarbonate...
2.6K
Multipotency and Niche of Bulge Stem Cell01:06

Multipotency and Niche of Bulge Stem Cell

4.3K
A hair follicle or HF is a small part of the skin that produces the hair shaft. Paul Gerson Unna was the first to observe a bulge in the human hair follicle's outer root sheath (ORS). The bulge is present between the sebaceous gland and the arrector pili muscle and is the niche for hair follicle stem cells (HFSCs). The bulge is also a niche for melanocyte stem cells, and their loss results in graying of hair. The HFSCs express Sox9 and Lhx2, which help them maintain stemness and prevent...
4.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Medullary cavity expansion is mediated by distinct cell populations during fetal bone development.

Nature communications·2026
Same author

Long-term inhibition of protease hypersensitivity by initial immunological cross-regulation and epigenetic memory in lung stromal cells.

Nature immunology·2026
Same author

Heparan sulfate binding protein treatment ameliorates neuropathology and behavioral abnormalities in mucopolysaccharidosis IIIB mice.

Cell death discovery·2025
Same author

Micro(nano)plastics: an Emerging Burden for Human Health.

International journal of biological sciences·2024
Same author

Morphological phenotyping of the aging cochlea in inbred C57BL/6N and outbred CD1 mouse strains.

Aging cell·2024
Same author

SGLT2 inhibitor promotes mitochondrial dysfunction and ER-phagy in colorectal cancer cells.

Cellular & molecular biology letters·2024

Related Experiment Video

Updated: Mar 9, 2026

Induced Differentiation of M Cell-like Cells in Human Stem Cell-derived Ileal Enteroid Monolayers
11:34

Induced Differentiation of M Cell-like Cells in Human Stem Cell-derived Ileal Enteroid Monolayers

Published on: July 26, 2019

9.2K

Neuronal VIP shapes intestinal stem cell activity and mucosal immunity.

Camilla Anastasio1, Lucie Peduto1

  • 1Stroma, Inflammation & Tissue Repair Unit, Institut Pasteur, Université Paris Cité, INSERM U1224, Paris, France.

Cell Stem Cell
|March 8, 2026
PubMed
Summary

Neuronal vasoactive intestinal peptide (VIP) acts as a brake on intestinal stem cells (ISCs) by signaling through VIPR1. This pathway limits intestinal regeneration and balances immune responses, crucial for gut homeostasis.

More Related Videos

Improved Swiss-rolling Technique for Intestinal Tissue Preparation for Immunohistochemical and Immunofluorescent Analyses
07:42

Improved Swiss-rolling Technique for Intestinal Tissue Preparation for Immunohistochemical and Immunofluorescent Analyses

Published on: July 13, 2016

73.7K
A Quantitative Cell Migration Assay for Murine Enteric Neural Progenitors
08:26

A Quantitative Cell Migration Assay for Murine Enteric Neural Progenitors

Published on: September 18, 2013

9.7K

Related Experiment Videos

Last Updated: Mar 9, 2026

Induced Differentiation of M Cell-like Cells in Human Stem Cell-derived Ileal Enteroid Monolayers
11:34

Induced Differentiation of M Cell-like Cells in Human Stem Cell-derived Ileal Enteroid Monolayers

Published on: July 26, 2019

9.2K
Improved Swiss-rolling Technique for Intestinal Tissue Preparation for Immunohistochemical and Immunofluorescent Analyses
07:42

Improved Swiss-rolling Technique for Intestinal Tissue Preparation for Immunohistochemical and Immunofluorescent Analyses

Published on: July 13, 2016

73.7K
A Quantitative Cell Migration Assay for Murine Enteric Neural Progenitors
08:26

A Quantitative Cell Migration Assay for Murine Enteric Neural Progenitors

Published on: September 18, 2013

9.7K

Area of Science:

  • Gastroenterology
  • Immunology
  • Stem Cell Biology

Background:

  • Intestinal homeostasis and regeneration are critically dependent on intestinal stem cells (ISCs).
  • Neuronal signals play a role in regulating ISC function and tissue repair.
  • The interplay between the nervous system and the intestinal immune system is vital for gut health.

Purpose of the Study:

  • To elucidate the role of neuronal vasoactive intestinal peptide (VIP) in regulating intestinal stem cell (ISC) activity.
  • To investigate the mechanism by which VIP influences intestinal regeneration and lineage expansion.
  • To understand how VIP-VIPR1 signaling impacts immune responses within the intestine.

Main Methods:

  • Utilized genetic models to study VIP and VIPR1 function in the intestine.
  • Employed lineage tracing and cell proliferation assays to assess ISC and secretory cell dynamics.
  • Analyzed immune cell populations and cytokine profiles in response to VIP signaling modulation.

Main Results:

  • Identified neuronal VIP as a key regulator that limits ISC proliferation and intestinal regeneration.
  • Demonstrated that VIP signaling through VIPR1 restrains the expansion of secretory lineages.
  • Showed that VIP-VIPR1 signaling contributes to balancing immune responses in the intestinal environment.

Conclusions:

  • Neuronal VIP acts as a crucial brake on intestinal stem cell-driven regeneration via VIPR1.
  • VIP-VIPR1 signaling is essential for controlling secretory cell numbers and maintaining immune homeostasis in the gut.
  • This pathway represents a significant target for therapeutic interventions in intestinal diseases.