Related Experiment Video
Updated: Mar 10, 2026

Principles of Rodent Surgery for the New Surgeon
Published on: January 6, 2011
How aseptic is aseptic technique conducted by nurses outside the operating room environment? A critical review
Edward Purssell1, Dinah Gould2
1Anglia Ruskin University, Chelmsford, UK; Little Havens Children's Hospice, Benfleet, UK.
Abstract:
Aseptic technique is integral to infection prevention and control, but there is no agreement concerning its aims, how it is undertaken outside operating rooms, and related terminology. In addition, there is a lack of concordance in international guidelines. This critical review was undertaken to explore breaches of asepsis that could occur during aseptic procedures outside of the operating room environment. There was little direct research evidence, leading to low levels of certainty in outcomes. This review established that low levels of contamination are exhibited by some materials not marketed as sterile. Contents of sterile procedure packs become contaminated rapidly after opening. Large, unwieldy procedure packs and the method of opening result in contamination. Surface contamination occurs as soon as colonized and infected wounds are exposed, and is persistent. Few studies have compared different approaches to conducting aseptic technique. Non-sterile disposable gloves may reduce the risk of microbial acquisition and transmission, but there are no data on how quickly sterile gloves become contaminated. In conclusion, there is a lack of evidence to underpin practice. Many non-sterile products are functionally sterile; conversely, sterile products become contaminated quickly. Relatively simple methods, such as the use of non-sterile disposable gloves and non-touch technique, could reduce the risk of microbial transmission significantly. Further research is needed to corroborate these findings.
More Related Videos
Related Concept Videos
Tracheostomy Care I: Pre-procedural Steps
Required Equipment
The equipment necessary for tracheostomy care includes:
Hand hygiene
Hand washing...
Asepsis
Suctioning the Oropharyngeal Airway
After assembling the equipment, the nurse should practice hand hygiene and don appropriate PPE according to infection control guidelines to avoid the...
Tracheostomy Suctioning II: Procedure
Sputum Studies II: Culture and Sensitivity
Sputum culture and sensitivity is a medical procedure used to diagnose bacterial infections in the respiratory tract and select the most appropriate antibiotics for treatment. This process involves analyzing sputum samples of thick and opaque secretions produced in the lungs and airways. These samples are collected from patients and then sent to the laboratory for analysis.
The test can identify various pathogens responsible for respiratory infections, including Streptococcus,...

