Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Plasticizers01:31

Plasticizers

Water-reducers, or plasticizers, are chemical admixtures used in concrete to improve strength and workability. These additives reduce the water-cement ratio without compromising workability, lower the cement content while maintaining the same workability, or increase workability to assist concrete placement in inaccessible areas.
Plasticizers function by using surface-active agents to create repulsive electrostatic forces between cement particles. This dispersion enhances the concrete's...
Pharmacokinetics in Obese Patients: Drug Metabolism and Excretion01:20

Pharmacokinetics in Obese Patients: Drug Metabolism and Excretion

Drug metabolism, a critical process in the liver, involves two primary phases: Phase I reactions and Phase II conjugation. Obesity introduces significant alterations in this metabolic process, primarily due to fatty infiltration of the liver, leading to conditions such as nonalcoholic fatty liver disease (NAFLD). This condition can modify the activities of both Phase I and II enzymes, impacting how drugs are metabolized in obese patients.Phase I metabolism sees variable effects across...
Bioplastics01:27

Bioplastics

Bioplastics derived from microbial processes present a sustainable alternative to conventional petroleum-based plastics. Among these, polyhydroxyalkanoates (PHAs), particularly polyhydroxybutyrates (PHBs), have emerged as prominent candidates due to their biodegradability and biocompatibility. These polymers are synthesized by a variety of bacteria, such as Cupriavidus necator and Pseudomonas putida, which naturally accumulate PHAs as intracellular carbon and energy reserves, especially under...
Microbial Bioremediation of Plastics01:28

Microbial Bioremediation of Plastics

Polyethylene terephthalate (PET) is a synthetic polymer widely utilized in the packaging industry, particularly for bottles and containers. Due to its chemical stability and durability, PET accumulates in the environment, contributing significantly to plastic pollution. It comprises repeating units of terephthalic acid and ethylene glycol, resulting in a semi-crystalline structure that is resistant to natural degradation processes.A notable breakthrough in plastic biodegradation came with the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Quercetin Mitigates Oxidative Stress-Induced Premature Senescence in SH-SY5Y Neuronal-like Cells.

International journal of molecular sciences·2026
Same author

Mapping emotional function in fibromyalgia: integrating alexithymia, pain catastrophizing, and self-compassion.

Therapeutic advances in musculoskeletal disease·2026
Same author

Differential Cadmium Responses in Two <i>Salvia</i> Species: Implications for Tolerance and Ecotoxicity.

Plants (Basel, Switzerland)·2026
Same author

Perfluorooctanoic acid affects in vitro adipogenesis in murine preadipocytes acting as an inducer of hypertrophic adipocytes.

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association·2026
Same author

Effects of Chlorogenic Acid on Cellular Senescence in an In Vitro Model of 3T3-L1 Murine Adipocytes.

Molecules (Basel, Switzerland)·2026
Same author

Phenolic Profile and In Vitro Anti-Inflammatory Activities of Salvia officinalis L. Hydrodistillation Wastewater.

Chemistry & biodiversity·2026

Related Experiment Video

Updated: Jul 23, 2026

Mechanism of Regulation of Adipocyte Numbers in Adult Organisms Through Differentiation and Apoptosis Homeostasis
08:34

Mechanism of Regulation of Adipocyte Numbers in Adult Organisms Through Differentiation and Apoptosis Homeostasis

Published on: June 3, 2016

15.9K

In vitro effects of polystyrene microplastics exposure on adipose tissue dysfunction.

Maria Sofia Molonia1, Santi Trischitta1, Federica Lina Salamone2

  • 1Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Viale F. Stagno D'Alcontres 31, 98166 Messina, Italy.

Toxicology in Vitro : an International Journal Published in Association with BIBRA
|March 9, 2026
PubMed
Summary

Polystyrene microplastics (MPs) did not affect mature fat cells. However, during differentiation, MPs caused fat cell growth, oxidative stress, and inflammation, highlighting stage-specific risks.

Keywords:
Adipose dysfunctionEndoplasmic reticulum stressObesogensPolystyrene microplastics

More Related Videos

Author Spotlight: Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator
06:08

Author Spotlight: Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator

Published on: May 19, 2023

3.0K
Isolation and Culturing of Primary Murine Adipocytes from Lean and Obese Mice
05:34

Isolation and Culturing of Primary Murine Adipocytes from Lean and Obese Mice

Published on: January 24, 2025

3.2K

Related Experiment Videos

Last Updated: Jul 23, 2026

Mechanism of Regulation of Adipocyte Numbers in Adult Organisms Through Differentiation and Apoptosis Homeostasis
08:34

Mechanism of Regulation of Adipocyte Numbers in Adult Organisms Through Differentiation and Apoptosis Homeostasis

Published on: June 3, 2016

15.9K
Author Spotlight: Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator
06:08

Author Spotlight: Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator

Published on: May 19, 2023

3.0K
Isolation and Culturing of Primary Murine Adipocytes from Lean and Obese Mice
05:34

Isolation and Culturing of Primary Murine Adipocytes from Lean and Obese Mice

Published on: January 24, 2025

3.2K

Area of Science:

  • Environmental Health
  • Cell Biology
  • Toxicology

Background:

  • Microplastics (MPs) are pervasive environmental contaminants.
  • Growing evidence links MPs to human health concerns.
  • Adipose tissue plays a crucial role in metabolic health.

Purpose of the Study:

  • To investigate the impact of polystyrene (PS) MPs on 3T3-L1 adipocytes.
  • To assess effects on both mature adipocytes and cells during differentiation.
  • To evaluate MPs' influence on adipogenesis, ER stress, oxidative balance, and inflammation.

Main Methods:

  • Utilized a murine 3T3-L1 adipocyte in vitro model.
  • Exposed differentiated adipocytes and differentiating preadipocytes to 5 μm PS MPs.
  • Analyzed key markers of adipogenesis, ER stress, inflammation, and oxidative stress.

Main Results:

  • PS MPs did not significantly alter mature adipocyte markers for adipogenesis, ER stress, or inflammation.
  • Exposure during differentiation promoted adipocyte hypertrophy and upregulated adipogenic markers.
  • MPs induced reactive oxygen species, ER stress, apoptosis, and inflammatory signaling.

Conclusions:

  • Adipose tissue response to PS MPs is stage-dependent.
  • MPs perturbing oxidative balance, ER homeostasis, and inflammatory signaling during differentiation.
  • Findings emphasize the need for stage-specific assessments of environmental contaminant impacts on adipose tissue.