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Updated: Mar 10, 2026

In Vitro Polymerization of F-actin on Early Endosomes
Published on: August 28, 2017
Endosomal actin attenuation and fission are regulated by MICAL2
Ajay B Murakonda1, Naava Naslavsky1, Steve Caplan1,2
1Department of Biochemistry & Molecular Biology, University of Nebraska Medical Center, Omaha, NE 68198, USA.
Abstract:
Endosome fission is essential for the generation of carrier vesicles and recycling of receptors to the plasma membrane. Early events in endosome fission depend on the generation of Arp2/3-mediated branched actin, which segregates cargo and constricts the endosomal membrane to form buds. It has been proposed that once membrane buds have been formed, branched actin attenuation is necessary for fission proteins, such as EHD1, to access the endosomal neck and complete the cleavage process. Whereas several proteins and complexes involved in actin growth at endosomes have been characterized, less is known about actin attenuation at endosomes. In our study, we identified MICAL2, a constitutively active actin-regulatory monooxygenase, as a key regulator of endosomal fission, likely through its regulation of branched actin. MICAL2 depletion, or inhibition of its monooxygenase activity, resulted in a substantial increase in branched actin associated with endosomes. Moreover, we demonstrate that MICAL2 is required for both endosome fission and the recycling of clathrin-dependent cargo. Overall, our study highlights a novel role for MICAL2 in regulating actin at endosomes, thereby facilitating fission and recycling.
Insights
MICAL2 regulates actin dynamics at endosomes, a crucial step for cell vesicle recycling. This study reveals MICAL2
Area of Science:
- Cell Biology
- Molecular Biology
- Biochemistry
Background:
- Endosome fission is vital for receptor recycling and carrier vesicle formation.
- Actin polymerization, specifically Arp2/3-mediated branched actin, drives early endosome budding.
- Actin attenuation is proposed to be necessary for the final fission step.
Purpose of the Study:
- To identify novel regulators of actin dynamics during endosome fission.
- To investigate the role of MICAL2 in endosomal membrane trafficking.
Main Methods:
- Depletion of MICAL2 using RNA interference.
- Inhibition of MICAL2's monooxygenase activity.
- Assessment of branched actin levels at endosomes via immunofluorescence.
- Analysis of endosome fission and clathrin-dependent cargo recycling.
Main Results:
- MICAL2 depletion or activity inhibition led to increased branched actin at endosomes.
- MICAL2 is essential for efficient endosome fission.
- MICAL2 is required for the proper recycling of clathrin-dependent cargo.
Conclusions:
- MICAL2 acts as a key regulator of actin dynamics at endosomes.
- MICAL2 facilitates endosome fission by modulating branched actin.
- MICAL2 plays a significant role in receptor recycling pathways.
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