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Updated: Jun 27, 2026

Vaccinia Virus Infection & Temporal Analysis of Virus Gene Expression: Part 1
Published on: April 8, 2009
Genomic comparison reveals single-nucleotide polymorphic sites and attenuation-associated site combinations specific
Yuchan Zhang1, Xuhua Duan1, Yuanyuan Cai1
1NMPA Center for Innovation and Research in Regulatory Science, Shanghai Quality Inspection and Testing Center of Innovative Biological Products, Shanghai Institute for Drug Control, Shanghai, China.
Abstract:
The live-attenuated varicella-zoster virus (VZV) vaccine, mainly based on the Oka strain, is essential for the prevention and control of chickenpox (varicella). Although genomic profiles of internationally prevalent Oka vaccine strain (vOka)-derived preparations have been reported, the comprehensive single-nucleotide polymorphism (SNP) profiles of multiple commercially available Chinese vOka-derived vaccines are still lacking. We performed next-generation sequencing (NGS) on five Chinese-manufactured VZV vaccines, comparing genomic variations against the wild-type parental Oka (pOka) strain and internationally prevalent vaccine products. Notably, among 54 key SNPs distinguishing Chinese vaccines from the wild-type pOka strain, 4 novel China-specific SNPs were identified. What's more, we identified 6 near-fixed (the vaccine allele frequency >90%) non-synonymous SNPs that consistently co-occurred across all production batches, potentially impacting protein function through amino acid substitutions. Genetic heterogeneity across manufacturers revealed manufacturer-specific signatures, whereas consistent batch production underscored manufacturing stability. By systematically mapping SNPs in Chinese VZV vaccines, this study bridges a critical knowledge gap and provides essential insights into manufacturer-specific genomic signatures.
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