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Updated: Jul 3, 2026

Cannulation of the Mouse Submandibular Salivary Gland via the Wharton's Duct
Published on: May 14, 2011
Technique for Difficult Wharton's Duct Cannulation
Evgeniya Molotkova1, Henry Hoffman1
1Department of Otolaryngology, Head and Neck Surgery University of Iowa Hospitals and Clinics Iowa City Iowa USA.
Abstract:
The comparative ease of cannulating Stensen's duct is contrasted by the acknowledged difficulty in cannulating Wharton's duct. Although access to Wharton's duct can be facilitated with a floor-of-mouth incision, it is desirable to avoid this open approach. We present a technique emphasizing the use of a tapered 4-0 Prolene suture to cannulate Wharton's duct. This guide directs placement of a 26-gauge angiocatheter through a modified Seldinger technique. The suture guide is then removed to permit infusion of saline with expansion of the duct to allow for passage of a 0.38-mm guidewire and progressive dilation with a 24- and 22-gauge angiocatheter over the larger guidewire. This technique was successful in all 4 patients who had difficult access to Wharton's duct. One of these patients also underwent successful cannulation of Bartholin's duct. This novel technique for cannulation of Wharton's duct offers an alternative approach for patients with challenging anatomy.
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