Open Porous Microenvironment-regulatory Microspheres Loaded with Curcumin@BSA NPs/BMSCs for Diabetic Wound Treatment

Area of Science:

• Biomaterials Science
• Regenerative Medicine
• Drug Delivery Systems

Background:

• Diabetic wound healing is impaired by chronic inflammation and hyperglycemia.
• Stem cell therapy and drug treatments show promise but face challenges like limited stem cell proliferation and drug availability.
• Developing advanced delivery systems is crucial for effective diabetic wound management.

Purpose of the Study:

• To design and synthesize open porous poly(lactic-co-glycolic acid) (PLGA) microspheres for sustained curcumin release.
• To investigate the efficacy of curcumin-loaded PLGA microspheres combined with bone marrow stem cells (BMSCs) in promoting diabetic wound healing.
• To evaluate the impact of this combination therapy on stem cell activity, inflammation, and tissue regeneration.

Main Methods:

• Poly(lactic-co-glycolic acid) (PLGA) microspheres were fabricated using gas-assisted volatilization microemulsion technology.
• Curcumin was encapsulated within the microspheres for sustained release over 35 days.
• The microspheres provided a supportive scaffold for bone marrow stem cells (BMSCs).

Main Results:

• Sustained curcumin release enhanced BMSC proliferation and maintained stem cell activity.
• The treatment modulated the wound immune microenvironment and alleviated inflammation via mitochondrial autophagy.
• Combined therapy promoted blood vessel regeneration and collagen network revitalization.

Conclusions:

• Curcumin-loaded PLGA microspheres effectively support stem cell therapy for diabetic wound healing.
• This integrated approach demonstrates significant potential for improving therapeutic outcomes in diabetic wound treatment.
• The developed microsphere system offers a promising strategy for addressing the challenges in diabetic wound regeneration.