Related Experiment Video
Updated: Mar 10, 2026
Probing the Brain in Autism Using fMRI and Diffusion Tensor Imaging
Published on: September 12, 2011
Analysis of NDNF and SLC1A2 gene expression in the dorsolateral prefrontal cortex of individuals with autism
Ariel Moraes de Andrade1, Larysy Raquelly Vidal de Souza2, Rodrigo Freire Oliveira1
1Laboratory of Experimental Neurology, Department of Biomedical Health, State University of Rio Grande do Norte, Mossoró, Brazil.
Introduction:
This study investigated the expression of NDNF and SLC1A2 in the dorsolateral prefrontal cortex of individuals with Autism Spectrum Disorder (ASD), a region linked to executive functions, emotional regulation, social skills, and sensory processing.
Methods:
Using data from the Allen Human Brain Atlas - Autism Study, 17 post-mortem cases (ASD: 9; controls: 8; ages 2-14) were analyzed with in situ hybridization and Nissl staining. Histological images were processed with FIJI-ImageJ and Ilastik software.
Results:
No significant differences in NDNF expression were observed, though a trend toward lower levels in ASD was noted. In contrast, SLC1A2 expression was significantly increased in ASD and showed age-related growth, possibly reflecting neuroinflammatory processes. Nissl staining indicated similar neuronal density between groups, suggesting that gene expression changes may reflect functional alterations rather than cell number.
Conclusion:
These results highlight complex neurogenesis alterations in ASD and underscore the need for further research to identify biomarkers and potential therapeutic targets.
