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Related Experiment Video

Updated: Mar 10, 2026

Detection of Viruses from Bioaerosols Using Anion Exchange Resin

Detection of Viruses from Bioaerosols Using Anion Exchange Resin

Published on: August 22, 2018

Aerosolization effects on coronavirus infectivity.

Meiyi Zhang¹, Gabriel L Hamer², Maria D King¹

¹Department of Biological and Agricultural Engineering, Texas A&M University, College Station, TX, United States.

Frontiers in Microbiology

|March 9, 2026

Summary

This summary is machine-generated.

Bovine coronavirus (BCoV) infectivity declined significantly with prolonged air mixing, while viral RNA persisted. Mechanical stress from air mixing, not nebulization, impacts airborne coronavirus viability, crucial for indoor environment infection control.

Keywords:

SARS-CoV-2 aerosolization aerosols bovine coronavirus nebulization virus infectivity

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Infection of Primary Nasal Epithelial Cells Grown at an Air-Liquid Interface to Characterize Human Coronavirus-Host Interactions

Infection of Primary Nasal Epithelial Cells Grown at an Air-Liquid Interface to Characterize Human Coronavirus-Host Interactions

Published on: September 22, 2023

Area of Science:

Virology
Infectious Disease Epidemiology
Environmental Health

Background:

Understanding airborne coronavirus persistence is vital for infection control.
The impact of aerosolization and airborne suspension on viral infectivity is not well-defined.

Purpose of the Study:

To evaluate how nebulization, aerosolization time, and air mixing affect bovine coronavirus (BCoV) viability and RNA persistence.
To use BCoV as a surrogate for human Betacoronaviruses to understand airborne transmission dynamics.

Main Methods:

BCoV suspensions were aerosolized using a Collison nebulizer in a mixed-air chamber.
Aerosols were collected with a Wetted Wall Cyclone (WWC).
Viral infectivity (TCID50) and RNA (qRT-PCR) were quantified in stock, nebulized suspensions, and collected aerosols.

Main Results:

Nebulization did not significantly impair BCoV viability or RNA.
WWC-collected aerosols showed a time-dependent decline in infectivity.
Viral RNA remained stable in aerosols, but decreased in the nebulizer reservoir initially.
Prolonged air mixing significantly reduced BCoV infectivity, while RNA persisted.

Conclusions:

Mechanical stress from continuous air mixing, common in indoor environments, significantly compromises airborne coronavirus infectivity.
Viral RNA is more stable than infectivity under prolonged airborne conditions.
Findings inform risk mitigation strategies for airborne viral transmission in indoor settings.