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Updated: Mar 10, 2026
An Oligonucleotide-based Tandem RNA Isolation Procedure to Recover Eukaryotic mRNA-Protein Complexes
Published on: August 18, 2018
Protocol to uncover the protein interactome of small non-coding vault RNAs through RNA antisense purification coupled
Sander B van der Kooij1, Jorn E Stok1, Annemarthe G van der Veen1
1Department of Immunology, Leiden University Medical Centre, Leiden, the Netherlands.
Abstract:
Vault RNAs (vtRNAs) are a family of four small non-coding RNAs (ncRNAs). Here, we present a protocol to identify protein interactors of vtRNA1-1, vtRNA1-2, and vtRNA1-3 in human lung epithelial cells through RNA antisense purification coupled to mass spectrometry (RAP-MS). We describe steps for using biotinylated vtRNA-specific antisense DNA probes to isolate vtRNA-protein complexes that are covalently crosslinked by treatment with ultraviolet (UV) light, followed by mass spectrometry-based analysis. This protocol can be adapted to other cells or small ncRNAs. For complete details on the use and execution of this protocol, please refer to Stok et al.1.
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