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Multimodal AFM-IR nanospectroscopy and non-linear optical microscopy for detecting collagen matrix alterations
Jérémie Mathurin1, Gaël Latour2,3, Gervaise Mosser4
1Institut de Chimie Physique, UMR 8000, Université Paris-Saclay, CNRS, 91405 Orsay, France. ariane.deniset@universite-paris-saclay.fr.
The Analyst
|March 9, 2026
Abstract:
Correlative photothermal infrared nanospectroscopy (AFM-IR) and non linear optical microscopy analyses reveal that the emergence of a 1730 cm-1 IR band in collagen arises from local, thermally induced esterification. This band serves as a marker of irreversible molecular alteration, associated with structural destabilisation and chemical changes within the collagen matrix.

