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Redox-Triggered Autologous Protein Assembly for Blood-Contacting Interfaces.

Mengjie Li¹, Yuhang Zhang², Yongchun Liu¹

¹Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, China.

ACS Applied Materials & Interfaces

|March 9, 2026

Summary

This summary is machine-generated.

Researchers developed a new method to create personalized, blood-contacting medical devices using a patient's own hemoglobin. This autologous-to-autologous (A-A) approach avoids contamination risks and enhances device functionality for biomedical applications.

Keywords:

Biocoating amyloid-like anticoagulation ligand dissociation surface modification

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Area of Science:

Biomaterials Science
Regenerative Medicine
Nanotechnology

Background:

Allogeneic (host-to-patient) bioresource applications face challenges with pathogen removal, immunogenicity, and contamination.
Existing methods often compromise the functional biomolecules within bioresources.
A need exists for safe and effective autologous (patient-to-self) bioresource utilization in biomedical devices.

Purpose of the Study:

To develop a novel strategy for creating hemoglobin-based ultrathin biocoatings using an autologous approach.
To enable the direct use of patient-derived hemoglobin for functionalizing medical devices.
To overcome the limitations of conventional bioresource processing for clinical translation.

Main Methods:

Introduced a ligand dissociation-induced phase-transition strategy for biocoating.
Utilized mild reductant-initiated, redox-triggered assembly of patient-derived hemoglobin.
Developed an autologous-to-autologous (A-A) route for constructing hemoglobin-based ultrathin biocoatings.
Demonstrated coassembly with heparin to form composite coatings.

Main Results:

Achieved efficient self-organization of hemoglobin into nanoscale films without harsh chemicals.
Enabled functionalization of diverse substrates (∼0.9 m² per 1 mL blood).
Created robust, antibiofouling coatings with enhanced hemocompatibility (prolonged activated partial thromboplastin time >600 s).
Demonstrated reduced systemic toxicity and eliminated cross-individual transmission risks.

Conclusions:

The developed strategy establishes a clinically translatable paradigm for personalized, blood-contacting medical devices.
This autologous hemoglobin-based biocoating approach offers a safe and effective alternative to allogeneic methods.
The technique enhances device functionality and hemocompatibility while mitigating risks associated with traditional bioresource applications.