Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Mar 10, 2026

Ultrasound Assessment of Endothelial Function: A Technical Guideline of the Flow-mediated Dilation Test
06:35

Ultrasound Assessment of Endothelial Function: A Technical Guideline of the Flow-mediated Dilation Test

Published on: April 27, 2016

18.9K

Guidelines for evaluating endothelial function in vascular tissue.

Cameron G McCarthy1, Christian Aalkjær2, Pooneh Bagher3

  • 1Department of Cell Biology and Anatomy, Cardiovascular Translational Research Center, School of Medicine, University of South Carolina, Columbia, South Carolina, United States.

American Journal of Physiology. Heart and Circulatory Physiology
|March 9, 2026
PubMed

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Building Resilience in Academic Science and Medicine.

Arteriosclerosis, thrombosis, and vascular biology·2026
Same author

Response to: Comment on "Plasma-based low-grade inflammation and endothelial dysfunction explain a small but significant proportion of the association between the n-3 polyunsaturated fatty acids and cardiovascular diseases: The Maastricht Study".

Journal of clinical lipidology·2026
Same author

Arterial iron regulates vasodilation during anemia via endothelial holo α-globin.

Blood·2026
Same author

Microbiome and cancer immunotherapy: a bibliometric analysis.

Journal of the Egyptian National Cancer Institute·2026
Same author

TRPM4 Couples Mechanical Force to Myogenic Constriction Throughout the Resistance Vasculature.

bioRxiv : the preprint server for biology·2026
Same author

Regional heterogeneity and sex differences along the mouse aorta.

American journal of physiology. Heart and circulatory physiology·2026
Same journal

Interplay of Oxidative Stress and Inflammatory Mechanisms in Doxorubicin-induced Cardiomyopathy.

American journal of physiology. Heart and circulatory physiology·2026
Same journal

Impact of moderate isocapnic hyperthermia on dynamic cerebral autoregulation and its directional sensitivity.

American journal of physiology. Heart and circulatory physiology·2026
Same journal

Repetitive Pressure Overload; Species Disparities, Mechanisms and Translational Relevance.

American journal of physiology. Heart and circulatory physiology·2026
Same journal

SIRT2-mediated regulation of glycolytic flux: Another brick in the wall of Gary Lopaschuk's legacy in cardiac metabolism.

American journal of physiology. Heart and circulatory physiology·2026
Same journal

Tailored anesthesia protocols for echo-based HFpEF phenotyping in C57BL6/N mice.

American journal of physiology. Heart and circulatory physiology·2026
Same journal

Desmoglein-2 Deficiency Drives Mitochondrial Morphological Remodeling in Cardiomyocytes.

American journal of physiology. Heart and circulatory physiology·2026
See all related articles
Summary
This summary is machine-generated.

This study provides guidelines for assessing endothelial function, integrating traditional and novel methods. These best practices aim to improve understanding and discovery in endothelial biology research.

Area of Science:

  • Cardiovascular Biology
  • Endothelial Cell Biology
  • Vascular Physiology

Background:

  • The endothelium is crucial for vascular homeostasis, regulating tone, inflammation, permeability, and thrombosis.
  • Understanding endothelial function is vital for cardiovascular health and disease research.
  • Existing methods for assessing endothelial function have limitations due to endothelial cell heterogeneity.

Purpose of the Study:

  • To establish consensus-based guidelines for evaluating endothelial function.
  • To integrate conventional functional assays with modern molecular techniques.
  • To promote methodological consistency and innovation in endothelial biology.

Main Methods:

  • Review and consensus development by leading experts in endothelial biology.
Keywords:
endothelial cellsendotheliummethodsvasodilationvessels

More Related Videos

A Methodological Approach to Non-invasive Assessments of Vascular Function and Morphology
09:33

A Methodological Approach to Non-invasive Assessments of Vascular Function and Morphology

Published on: February 7, 2015

16.9K
Assessing Endothelial Vasodilator Function with the Endo-PAT 2000
07:46

Assessing Endothelial Vasodilator Function with the Endo-PAT 2000

Published on: October 15, 2010

44.6K

Related Experiment Videos

Last Updated: Mar 10, 2026

Ultrasound Assessment of Endothelial Function: A Technical Guideline of the Flow-mediated Dilation Test
06:35

Ultrasound Assessment of Endothelial Function: A Technical Guideline of the Flow-mediated Dilation Test

Published on: April 27, 2016

18.9K
A Methodological Approach to Non-invasive Assessments of Vascular Function and Morphology
09:33

A Methodological Approach to Non-invasive Assessments of Vascular Function and Morphology

Published on: February 7, 2015

16.9K
Assessing Endothelial Vasodilator Function with the Endo-PAT 2000
07:46

Assessing Endothelial Vasodilator Function with the Endo-PAT 2000

Published on: October 15, 2010

44.6K
  • Integration of traditional techniques (e.g., myography) with emerging methodologies (e.g., transcriptomics, proteomics, advanced imaging).
  • Guidelines cover endothelial cell cultures and isolated vascular tissues.

    • Main Results:

    • Consensus reached on widely used techniques for endothelial function assessment.
    • Establishment of best practices for evaluating endothelial function across various models.
    • Guidelines emphasize combining functional assays with molecular approaches.

    Conclusions:

    • Adoption of these guidelines will enhance rigor and reproducibility in endothelial research.
    • Methodological consistency and innovation are key to advancing endothelial biology.
    • Improved understanding of endothelial cell diversity and function in health and disease is anticipated.