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Distinct TRAPP complexes activate Ypt/Rab GTPases in secretion and autophagy.

Valeriya Gyurkovska1, Rakhilya Murtazina1, Sarah F Zhao1

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TRAPP complexes are activators of Ypt/Rab GTPases in intracellular transport. This study clarifies TRAPP I and II roles in early and late secretion, respectively, and TRAPP III in autophagy, resolving controversies in Ypt/Rab regulation.

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Ypt/Rab GTPases are key regulators of intracellular transport.
  • TRAPP complexes activate Ypt/Rab GTPases, but their specificities are debated.
  • Ypt1/Rab1 and Ypt31/Rab11 are involved in secretion and autophagy.

Purpose of the Study:

  • To determine the specificities of yeast TRAPP complexes in regulating Ypt/Rab GTPases.
  • To elucidate the roles of TRAPPI, TRAPPII, and TRAPPIII in intracellular transport pathways.

Main Methods:

  • In vivo analyses of mutations in yeast TRAPP complex subunits.
  • Conditional depletion of essential TRAPPI and TRAPPIII subunits.
  • Assessment of Golgi transport and protein localization.

Main Results:

  • Deletion of Trs85 (TRAPPIII-specific) did not affect Golgi transport or Ypt1 localization.
  • TRAPPI and TRAPPII are essential for early and late secretion steps, respectively.
  • TRAPPI and TRAPPII activate Ypt1 and Ypt31 in early and late Golgi, respectively.
  • TRAPPIII is implicated in Ypt1 activation specifically in autophagy.

Conclusions:

  • TRAPPI and TRAPPII exhibit distinct specificities for Ypt/Rab activation in secretion.
  • TRAPPIII's role is specific to Ypt1 activation during autophagy.
  • This provides a mechanistic basis for the dual role of Ypt1/Rab1 in secretion and autophagy.