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Updated: Mar 11, 2026

A Scanning Electron Microscopy-Compatible Optical Imaging Method for Mesoscopic All-Cell Brain Mapping

A Scanning Electron Microscopy-Compatible Optical Imaging Method for Mesoscopic All-Cell Brain Mapping

Published on: February 20, 2026

A Scanning Electron Microscopy-Compatible Optical Imaging Method for Mesoscopic All-Cell Brain Mapping.

Zhongyang Li¹, Peiyao Shi², Ruobing Zhang³

¹School of Biomedical Engineering (Suzhou), Division of Life Sciences and Medicine, University of Science and Technology of China; Anhui Province Key Laboratory of Biomedical Imaging and Intelligent Processing, Institute of Artificial Intelligence, Hefei Comprehensive National Science Center; Department of Biomedical Engineering, City University of Hong Kong.

Journal of Visualized Experiments : Jove

|March 9, 2026

Summary

This summary is machine-generated.

Optical Multilayer Interference Tomography (OMLIT) enables comprehensive brain atlas construction by indiscriminately imaging all cells. This technique integrates with electron microscopy, improving efficiency for multi-scale neural network studies.

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Last Updated: Mar 11, 2026

A Scanning Electron Microscopy-Compatible Optical Imaging Method for Mesoscopic All-Cell Brain Mapping

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Published on: February 20, 2026

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Focussed Ion Beam Milling and Scanning Electron Microscopy of Brain Tissue

Focussed Ion Beam Milling and Scanning Electron Microscopy of Brain Tissue

Published on: July 6, 2011

Area of Science:

Neuroscience
Biophysics
Imaging Technology

Background:

Constructing detailed brain atlases requires imaging techniques with both broad field of view and subcellular resolution.
Current optical and electron microscopy methods have limitations in imaging all cells within a single specimen.

Purpose of the Study:

Introduce Optical Multilayer Interference Tomography (OMLIT) for indiscriminate optical imaging of all cells in electron microscopy-prepared brain specimens.
Enable the reconstruction of complete brain atlases encompassing all neural cells.
Facilitate seamless integration of OMLIT with automated tape-collecting ultramicrotomy scanning electron microscopy (ATUM-SEM) workflows.

Main Methods:

Developed and validated Optical Multilayer Interference Tomography (OMLIT) for brain specimen imaging.
Integrated OMLIT with automated tape-collecting ultramicrotomy scanning electron microscopy (ATUM-SEM).
Applied the combined technique to adult mouse cerebral cortex samples.

Main Results:

OMLIT enables indiscriminate optical imaging of all cells in specimens prepared for electron microscopy.
OMLIT imaging provides mesoscale structural information prior to electron microscopy.
Successful validation in adult mouse cerebral cortex samples demonstrates method compatibility and accuracy.

Conclusions:

OMLIT is a novel imaging technique for reconstructing complete brain atlases of all neural cells.
The integration of OMLIT with ATUM-SEM streamlines multi-scale brain atlas construction.
This method significantly reduces the area and data volume for high-resolution electron microscopy imaging, enhancing research efficiency.