Lacticaseibacillus paracasei KBL382 contributes to the immunomodulation in THP-1 cells.

Area of Science:

• Immunology
• Microbiology
• Gastroenterology

Background:

• Gut microbiome dysbiosis can trigger inflammation through Toll-like receptor 2 (TLR2) pathways.
• Lactobacillus species are recognized for their potent immunomodulatory properties and probiotic applications.

Purpose of the Study:

• To investigate the immunomodulatory effects of live and pasteurized Lacticaseibacillus paracasei KBL382 in vitro.
• To assess the impact of L. paracasei KBL382 on key inflammatory signaling molecules in a human monocytic cell model.

Main Methods:

• Utilized an in vitro monocytic THP-1 cell model.
• Stimulated cells with live or pasteurized L. paracasei KBL382, with and without lipopolysaccharide (LPS).
• Quantified the expression levels of TLR2, MyD88, IRAK1, IL-6, IL-10, IL-12, A20, SOCS1, and IRAK3.

Main Results:

• Live L. paracasei KBL382 significantly upregulated TLR2, MyD88, and IRAK1 expression, independent of LPS.
• Live L. paracasei KBL382 increased interleukin (IL)-6 and IL-10 levels under LPS stimulation.
• Live L. paracasei KBL382 elevated the expression of negative inflammation regulators A20, SOCS1, and IRAK3.
• Pasteurized L. paracasei decreased IL-12 levels.

Conclusions:

• L. paracasei KBL382 demonstrates significant immunomodulatory capacity by balancing pro-inflammatory and anti-inflammatory signaling pathways.
• This probiotic strain effectively modulates immune responses through the gut-immune axis.
• L. paracasei KBL382 is a promising candidate for mitigating chronic inflammation.