Related Experiment Video

Updated: Mar 11, 2026

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens
13:03

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens

Published on: March 8, 2018

11.2K

High-Quality Genome Assemblies of Two Prototheca wickerhamii Strains

Lihua Fang1, Jian Guo2, Qing Ning3

  • 1Department of Endocrinology, Shenzhen Longhua District Central Hospital, Shenzhen, 518110, China.

Scientific Data
|March 10, 2026
PubMed

Insights

High-quality genome assemblies for Prototheca wickerhamii strains Pw26 and PwS1 were generated. This provides a valuable resource for understanding this opportunistic pathogen and its genomic features.

Area of Science:

  • Microbiology
  • Genomics
  • Medical Mycology

Background:

  • Prototheca wickerhamii is a non-photosynthetic microalgal species.
  • This species is known to cause opportunistic human infections.
  • Understanding its genomic characteristics is vital for medical and symbiosis research.

Purpose of the Study:

  • To generate high-quality genome assemblies for two strains of Prototheca wickerhamii (Pw26 and PwS1).
  • To provide a genomic resource for comparative genomics and functional studies of P. wickerhamii.

Main Methods:

  • Genome sequencing using PacBio HiFi reads.
  • Assembly and evaluation of genome completeness and accuracy using BUSCO analysis.
  • Comparative genomic analysis of genome size, gene content, and repeat sequences.

Main Results:

  • High-quality genome assemblies for Pw26 (17.8 MB) and PwS1 (17.4 MB) were generated.
  • Both genomes exhibited a GC content of 63.5% and high contig N50 values (1.6 MB).
  • Comparative analysis revealed high similarity in genome size and alignment, with Pw26 having slightly more protein-coding genes.

Conclusions:

  • The generated genome assemblies are valuable resources for Prototheca wickerhamii research.
  • Detailed genomic characterization facilitates further studies into pathogenic mechanisms.
  • These findings support comparative genomics and functional exploration of this microalgal species.

More Related Videos

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies12:08

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

Published on: August 20, 2021

5.9K
Purification of High Molecular Weight Genomic DNA from Powdery Mildew for Long-Read Sequencing06:56

Purification of High Molecular Weight Genomic DNA from Powdery Mildew for Long-Read Sequencing

Published on: March 31, 2017

12.3K

Related Concept Videos

Genome Annotation and Assembly03:36

Genome Annotation and Assembly

The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
21.3K
Genomic DNA in Prokaryotes00:46

Genomic DNA in Prokaryotes

The genome of most prokaryotic organisms consists of double-stranded DNA organized into one circular chromosome in a region of cytoplasm called the nucleoid. The chromosome is tightly wound, or supercoiled, for efficient storage. Prokaryotes also contain other circular pieces of DNA called plasmids. These plasmids are smaller than the chromosome and often carry genes that confer adaptive functions, such as antibiotic resistance.
Genomic Diversity in Bacteria
Although bacterial genomes are much...
49.7K