Polysaccharides From Bombax ceiba Flower by Deep Eutectic Solvent Based Three-Phase Partitioning and Their Protective...

Area of Science:

• Natural Product Chemistry
• Pharmacology
• Green Chemistry

Background:

• Bombax ceiba flower polysaccharides (BCFP) possess potential bioactivities.
• Efficient and eco-friendly extraction methods are needed for bioactive compounds.
• Ulcerative colitis (UC) is a chronic inflammatory condition requiring novel therapeutic strategies.

Purpose of the Study:

• To develop a green and efficient deep eutectic solvent (DES)-based three-phase partitioning (TPP) method for BCFP extraction.
• To characterize the extracted BCFP.
• To evaluate the therapeutic potential of BCFP in a dextran sulfate sodium (DSS)-induced UC mouse model.

Main Methods:

• Optimized DES-1 (lauric acid and decanoic acid) for TPP extraction of BCFP.
• Characterized BCFP's monosaccharide composition, molecular weight, and acidic heteropolysaccharide nature.
• Administered BCFP to DSS-induced UC mice to assess its effects on disease activity, inflammation, oxidative stress, and intestinal barrier function.

Main Results:

• Optimal BCFP extraction yield of 13.53% achieved using specific DES-1 conditions.
• BCFP is an acidic heteropolysaccharide with an average molecular weight of 2.15 kDa, composed of various monosaccharides.
• BCFP administration significantly ameliorated DSS-induced UC symptoms, reduced inflammatory cytokines, oxidative stress, and improved intestinal barrier integrity.

Conclusions:

• A cost-effective and environmentally friendly DES-based TPP method was established for BCFP extraction.
• BCFP exhibits significant anti-inflammatory and gut-protective properties.
• BCFP holds promise as a functional food ingredient or pharmaceutical agent for colitis management.