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Updated: Mar 11, 2026

Drug Treatment and In Vivo Imaging of Osteoblast-Osteoclast Interactions in a Medaka Fish Osteoporosis Model
Published on: January 1, 2017
Conformational Dynamics and Osteoclastogenesis Inhibition by Cladiellin-Type Diterpenes from the Marine Gastropod
Nozomi Shiiba1, Akana Matsuda2, Tomoki Tsuruta3
1Graduate School of Agriculture, Forestry and Fisheries, Kagoshima University, Shimoarata 4-50-20, Kagoshima 890-0056, Japan.
Abstract:
A cladiellin-type diterpene, 3-O-deacetylcladiellin (1), was isolated from the marine gastropod Ovula ovum, together with a known analogue 2. The NMR spectra of 1 and 2 exhibited unusually broad resonances. The structure and absolute configuration of 1 were unambiguously established by X-ray crystallographic analysis and electronic circular dichroism spectroscopy. DFT-based conformational analysis combined with variable-temperature NMR revealed that the NMR coalescence arises from conformational exchange of the flexible nine-membered ring. Chemical evidence suggests that 1 is likely derived from an unidentified soft coral prey of O. ovum. Compound 1 inhibits RANKL-induced osteoclast differentiation in RAW264 cells.
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