Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Stem Cell Therapy for Tissue Regeneration01:21

Stem Cell Therapy for Tissue Regeneration

4.8K
Stem cell therapy is a method used in regenerative medicine to repair and restore function to damaged tissues and organs. Stem cells have the potential to proliferate and differentiate into various tissue types, making them ideal candidates for tissue regeneration. For example, hematopoietic stem cell transplants are commonly used in blood cancer treatment to replenish damaged bone marrow and restore healthy blood cells.
Types of Stem Cells used in Stem Cell Therapy
The two main cell...
4.8K
iPS Cell Differentiation01:22

iPS Cell Differentiation

3.2K
The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.
3.2K
Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

28.3K
Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
28.3K
Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

5.8K
Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
5.8K
Stem Cell Culture01:17

Stem Cell Culture

6.3K
Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...
6.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mitochondrial DNA heteroplasmy drives cortical neuronal disturbances in human organoids harbouring the common m.3243A>G mutation.

Nature communications·2026
Same author

Soft-shell phacoemulsification in Fuchs' endothelial dystrophy: Central corneal thickness as a predictor of post-operative keratoplasty.

European journal of ophthalmology·2026
Same author

Retinal organoid screening reveals ABT-737 and luminespib as potential agents against a cone- precursor-derived subtype of retinoblastoma.

Molecular therapy. Oncology·2026
Same author

Shaping the next era of stem cell science: a vision for stem cells journal and its global community.

Stem cells (Dayton, Ohio)·2025
Same author

IVCM image analysis for limbal stem cell deficiency: quantitative diagnostics of the corneal epithelium post-transplant recovery.

The ocular surface·2025
Same author

Prospective consecutive case series of patients with neurotrophic keratopathy associated with unilateral total limbal stem cell deficiency caused by severe ocular surface burns.

The ocular surface·2025
Same journal

Industry updates in advanced therapy medicinal products and regenerative medicine - May 2026.

Regenerative medicine·2026
Same journal

Ethical, legal, and social issues associated with human fetal tissue research in Japan.

Regenerative medicine·2026
Same journal

The future of hematopoietic stem cell and stem cell gene therapy for metabolic diseases.

Regenerative medicine·2026
Same journal

Exploring gene therapy for developmental and epileptic encephalopathies (DEEs): possibilities or promises?

Regenerative medicine·2026
Same journal

Industry updates in advanced therapy medicinal products and regenerative medicine - April 2026.

Regenerative medicine·2026
Same journal

Optimizing strategies in tendon tissue engineering through effective scaffold design: overview of recent advancements.

Regenerative medicine·2026
See all related articles

Related Experiment Video

Updated: Mar 11, 2026

A Simple Mechanical Procedure to Create Limbal Stem Cell Deficiency in Mouse
04:55

A Simple Mechanical Procedure to Create Limbal Stem Cell Deficiency in Mouse

Published on: November 17, 2016

8.8K

Implementing stem-cell therapies for limbal stem cell deficiency.

Eldo Galo1, Francisco C Figueiredo1,2, Majlinda Lako1

  • 1Biosciences Institute, Newcastle University, Newcastle Upon Tyne, UK.

Regenerative Medicine
|March 10, 2026
PubMed
Summary
This summary is machine-generated.

Limbal stem cell deficiency (LSCD) treatments are advancing, but outcomes vary. Future research focuses on better stem cell characterization and universal donor cells to improve efficacy for this challenging eye condition.

Keywords:
CorneaLSCDLSCscell therapycorneal epithelium

More Related Videos

Author Spotlight: Standardizing Limbal Niche Cell (LNC) Isolation and Characterization to Support Widespread LNC Research
10:11

Author Spotlight: Standardizing Limbal Niche Cell (LNC) Isolation and Characterization to Support Widespread LNC Research

Published on: October 27, 2023

1.6K
Efficient and Scalable Directed Differentiation of Clinically Compatible Corneal Limbal Epithelial Stem Cells from Human Pluripotent Stem Cells
10:07

Efficient and Scalable Directed Differentiation of Clinically Compatible Corneal Limbal Epithelial Stem Cells from Human Pluripotent Stem Cells

Published on: October 24, 2018

8.3K

Related Experiment Videos

Last Updated: Mar 11, 2026

A Simple Mechanical Procedure to Create Limbal Stem Cell Deficiency in Mouse
04:55

A Simple Mechanical Procedure to Create Limbal Stem Cell Deficiency in Mouse

Published on: November 17, 2016

8.8K
Author Spotlight: Standardizing Limbal Niche Cell (LNC) Isolation and Characterization to Support Widespread LNC Research
10:11

Author Spotlight: Standardizing Limbal Niche Cell (LNC) Isolation and Characterization to Support Widespread LNC Research

Published on: October 27, 2023

1.6K
Efficient and Scalable Directed Differentiation of Clinically Compatible Corneal Limbal Epithelial Stem Cells from Human Pluripotent Stem Cells
10:07

Efficient and Scalable Directed Differentiation of Clinically Compatible Corneal Limbal Epithelial Stem Cells from Human Pluripotent Stem Cells

Published on: October 24, 2018

8.3K

Area of Science:

  • Ophthalmology
  • Regenerative Medicine
  • Stem Cell Biology

Background:

  • Limbal stem cell deficiency (LSCD) is a severe ocular surface disorder posing significant therapeutic challenges, especially in bilateral cases.
  • Current treatments have evolved over decades, yet long-term outcomes remain inconsistent due to factors like disease severity and immune rejection.

Purpose of the Study:

  • To review the current landscape of surgical and stem cell-based therapies for LSCD.
  • To highlight clinical outcomes, limitations, and translational challenges of existing and emerging treatments.
  • To discuss future directions for enhancing LSCD therapy efficacy and accessibility.

Main Methods:

  • Review of current surgical and stem cell-based interventions for LSCD.
  • Examination of autologous and allogeneic limbal epithelial transplantation, simple limbal epithelial transplantation, oral mucosa-derived approaches, mesenchymal stem cell therapies, and induced pluripotent stem cell-derived corneal epithelium.
  • Analysis of clinical outcomes, biological limitations, and translational hurdles.

Main Results:

  • Stem cell-based interventions have transformed LSCD management, but clinical outcomes are variable.
  • Key influencing factors include disease severity, immune rejection, stem cell quality, and ocular surface microenvironment optimization.
  • Various approaches are being explored, including transplantation, oral mucosa grafts, MSCs, and iPSC-derived corneal epithelium.

Conclusions:

  • Improving LSCD therapy requires better stem cell characterization and development of hypoimmune or universal donor cell products.
  • Addressing biological limitations and translational challenges is crucial for enhancing therapeutic efficacy.
  • Future directions aim to improve accessibility and long-term success rates for patients with LSCD.