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Updated: Mar 12, 2026
A Quantitative Detection Method for MicroRNAs in the Kidney of an Ischemic Kidney Injury Mouse Model
Published on: September 11, 2020
MicroRNAs miR-29a-3p and miR-192-5p: promising urinary biomarkers for kidney function loss
Cristine Dieter1,2,3, Eliandra Girardi1, Marcia Puñales4
1Hospital de Clínicas de Porto Alegre Serviço de Endocrinologia Porto Alegre RG Brasil Serviço de Endocrinologia, Hospital de Clínicas de Porto Alegre, Porto Alegre, RG, Brasil.
Objective:
This study aimed to evaluate the expression of miR-29a-3p and miR-192-5p in patients with type 1 diabetes mellitus (T1DM) with diabetic kidney disease (DKD) compared to those without DKD.
Subjects And Methods:
This study included 29 patients with T1DM, comprising 13 without DKD (non-DKD group) and 16 with DKD, who were further subdivided into nine patients with moderate DKD and seven with severe DKD. MiR-29a-3p and miR-192-5p expression levels were measured in urine samples using qPCR and are presented as medians (25-75th percentiles).
Results:
miR-29a-3p levels were higher in patients with DKD compared to the non-DKD group [1.24 (0.97-1.74) versus 0.83 (0.72-0.99); P = 0.008]. Its expression showed a negative correlation with estimated glomerular filtration rate (eGFR) (P = 0.007) and a positive correlation with creatinine levels (P = 0.004). MiR-192-5p levels were higher in patients with moderate DKD compared to the non-DKD group [2.15 (1.45-4.21) versus vs. 1.42 (0.98-2.45); P = 0.015], showing a negative correlation with eGFR (P = 0.003) and a positive correlation with creatinine (P = 0.006).
Conclusion:
The differential expression of miR-29a-3p and miR-192-5p in DKD highlights their potential as promising biomarkers for this complication.
Insights
Higher levels of miR-29a-3p and miR-192-5p were found in patients with diabetic kidney disease (DKD). These microRNAs may serve as biomarkers for DKD in type 1 diabetes mellitus (T1DM).
Area of Science:
- Biochemistry
- Molecular Biology
- Nephrology
Background:
- Diabetic kidney disease (DKD) is a major complication of type 1 diabetes mellitus (T1DM).
- Identifying reliable biomarkers for DKD is crucial for early diagnosis and management.
- MicroRNAs (miRNAs) have emerged as potential diagnostic and prognostic indicators in various diseases.
Purpose of the Study:
- To evaluate the urinary expression levels of miR-29a-3p and miR-192-5p.
- To compare miRNA expression between T1DM patients with and without DKD.
- To investigate the correlation between miRNA expression and indicators of kidney function.
Main Methods:
- The study included 29 T1DM patients, categorized into non-DKD and DKD groups.
- Urinary miRNA expression was quantified using quantitative polymerase chain reaction (qPCR).
- Statistical analyses were performed to compare groups and assess correlations.
Main Results:
- Urinary miR-29a-3p levels were significantly higher in patients with DKD compared to those without.
- Urinary miR-192-5p levels were elevated in patients with moderate DKD.
- Both miRNAs showed negative correlations with estimated glomerular filtration rate (eGFR) and positive correlations with creatinine levels.
Conclusions:
- Differential expression of miR-29a-3p and miR-192-5p suggests their involvement in DKD pathogenesis.
- These miRNAs hold promise as potential non-invasive biomarkers for detecting DKD in T1DM patients.
- Further research is warranted to validate their clinical utility.
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