Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

An interpretable early intensive care unit model for predicting in-hospital mortality in trauma patients combined with rib fractures: development and external validation.

Journal of thoracic disease·2026
Same author

Association of tumor associated collagen signature with lymph node metastasis in pancreatic ductal adenocarcinoma.

Bioengineering & translational medicine·2026
Same author

Denoising-Assisted Rapid Multiphoton Imaging for Analysing Traumatic Penumbra Microenvironment in Rat Brain.

Journal of biophotonics·2026
Same author

Achieving Near-Lossless Adsorption Capacity via Hierarchical All-Fibrous Strategy for Efficient Lithium Extraction.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

An Imported Case of Dengue/Zika Coinfection - Sichuan Province, China, 2026.

China CDC weekly·2026
Same author

From Label-Free Multiphoton Imaging to Pathological Reports: A Vision-Language Breast Cancer Margin Pathological Diagnosis System.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Related Experiment Video

Updated: Mar 12, 2026

A Label-Free Segmentation Approach for Intravital Imaging of Mammary Tumor Microenvironment
10:39

A Label-Free Segmentation Approach for Intravital Imaging of Mammary Tumor Microenvironment

Published on: May 24, 2022

2.8K

Label-Free Identification of Choroidal Melanoma Infiltration Boundaries Using Multiphoton Microscopy Combined With

Linjing Shi1, Liwen Hu2, Linghan You1

  • 1School of Medical Technology and Engineering, Fujian Medical University, Fuzhou, China.

Journal of Biophotonics
|March 11, 2026
PubMed
Summary
This summary is machine-generated.

Multiphoton microscopy (MPM) offers label-free identification of choroidal melanoma boundaries by analyzing NADH and collagen alterations. This technique promises enhanced surgical precision for tumor resection.

Keywords:
choroidal melanomamultiphoton microscopysecond harmonic generationtwo‐photon excitation fluorescence

More Related Videos

Implantation and Evaluation of Melanoma in the Murine Choroid via Optical Coherence Tomography
05:46

Implantation and Evaluation of Melanoma in the Murine Choroid via Optical Coherence Tomography

Published on: December 2, 2022

2.2K
DNA-barcode-based Multiplex Immunofluorescence Imaging to Analyze FFPE Specimens from Genetically Reprogrammed Murine Melanoma
09:58

DNA-barcode-based Multiplex Immunofluorescence Imaging to Analyze FFPE Specimens from Genetically Reprogrammed Murine Melanoma

Published on: June 6, 2025

1.2K

Related Experiment Videos

Last Updated: Mar 12, 2026

A Label-Free Segmentation Approach for Intravital Imaging of Mammary Tumor Microenvironment
10:39

A Label-Free Segmentation Approach for Intravital Imaging of Mammary Tumor Microenvironment

Published on: May 24, 2022

2.8K
Implantation and Evaluation of Melanoma in the Murine Choroid via Optical Coherence Tomography
05:46

Implantation and Evaluation of Melanoma in the Murine Choroid via Optical Coherence Tomography

Published on: December 2, 2022

2.2K
DNA-barcode-based Multiplex Immunofluorescence Imaging to Analyze FFPE Specimens from Genetically Reprogrammed Murine Melanoma
09:58

DNA-barcode-based Multiplex Immunofluorescence Imaging to Analyze FFPE Specimens from Genetically Reprogrammed Murine Melanoma

Published on: June 6, 2025

1.2K

Area of Science:

  • Ophthalmology
  • Biomedical Optics
  • Surgical Oncology

Background:

  • Choroidal melanoma excision relies on subjective visual assessment.
  • Improved intraoperative guidance is needed for precise tumor removal.

Purpose of the Study:

  • To evaluate multiphoton microscopy (MPM) for label-free characterization of choroidal melanoma boundaries.
  • To assess spectral and microstructural features for differentiating tumor from normal tissue.

Main Methods:

  • Multiphoton microscopy (MPM) in lambda mode was used to analyze spectral differences.
  • Image processing techniques quantified collagen parameters for structural analysis.
  • NADH molecular changes were investigated for qualitative differentiation.

Main Results:

  • MPM enabled label-free identification of the tumor boundary.
  • NADH molecular changes provided a basis for qualitative differentiation.
  • Collagen fiber alterations offered a potential quantitative index for boundary identification.

Conclusions:

  • MPM is capable of identifying choroidal melanoma boundaries.
  • MPM provides label-free, precise characterization of tumor margins.
  • Advancements in multiphoton endoscopes may enable intraoperative guidance for melanoma resection.