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Published on: September 10, 2016
A cold-active β-galactosidase from kimchi-derived Latilactobacillus sakei J15: Enzyme properties and probiotic
Ruya Tan1, Enze Zhang1, Yaxuan Li1
1School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023 Zhejiang, China.
Background:
Latilactobacillus sakei (Lb. sakei) could be isolated from various fermented foods, that could adapted under low-temperature conditions.
Objective:
This study investigated the potential probiotic properties of Lb. sakei J15, a strain isolated from kimchi that exhibits high β-galactosidase activity at low temperatures.
Methods:
The growth characteristics of Lb. sakei J15 under different temperatures, salt concentrations, and carbon sources were investigated.
Results:
These results showed that Lb. sakei J15 exhibited the highest β-galactosidase activity (789.31 mU/mg) at a low temperature (15 °C), along with high survival rates under acidic (pH 3.0) and bile salt (0.3%) conditions, reaching 91.5% and 66.0%, respectively. Additionally, Lb. sakei J15 demonstrated strong tolerance in a simulated gastrointestinal digestion system, with survival rates of 63.0% after 3 h of gastric digestion and 47.0% after 8 h of intestinal digestion. The strain also exhibited notable antioxidant activity, with DPPH and ABTS radical scavenging rates of 43.0% and 71.9%, respectively, as well as a high auto-aggregation ability (96.1%). Furthermore, Lb. sakei J15 effectively utilized fructooligosaccharides (FOS) and galactooligosaccharides (GOS) as carbon sources. It also showed high lysozyme tolerance (93.7% survival rate) and displayed no hemolytic activity.
Conclusions:
In conclusion, Lb. sakei J15 showed potential as a starter culture for low-temperature dairy fermentation.
Highlights:
Lb. sakei J15 had potential probiotc effects.
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