Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A fatty acid amide activates myeloid cells and improves neurovascular outcomes in retinal degeneration.

Nature neuroscience·2026
Same author

Genetic and pharmacological inactivation of peptidoglycan remodeling increases antibiotic susceptibility of vancomycin-resistant Enterococcus faecium.

Nature communications·2026
Same author

A Global Ligandability Map of Tryptoline Butynamide Stereoprobes Identifies Covalent Inhibitors of the Actin Maturation Protease.

Journal of the American Chemical Society·2026
Same author

Genetic and pharmacological inactivation of peptidoglycan remodeling increases antibiotic susceptibility of vancomycin-resistant <i>Enterococcus faecium</i>.

bioRxiv : the preprint server for biology·2026
Same author

High-throughput ligand diversification to discover chemical inducers of proximity.

Nature chemical biology·2026
Same author

Cysteine Reactivity Profiling Identifies Host Regulators of <i>Mycobacterium tuberculosis</i> Replication in Human Macrophages.

ACS infectious diseases·2026

Related Experiment Video

Updated: Mar 12, 2026

Dissecting Cell-Autonomous Function of Fragile X Mental Retardation Protein in an Auditory Circuit by In Ovo Electroporation
11:10

Dissecting Cell-Autonomous Function of Fragile X Mental Retardation Protein in an Auditory Circuit by In Ovo Electroporation

Published on: July 6, 2022

2.7K

Dissecting FOXA1 pioneering function by acute pharmacological degradation.

Lauren M Hargis, Paige A Barta, Yuxiang Zhang

    Biorxiv : the Preprint Server for Biology
    |March 11, 2026
    PubMed
    Summary

    Pioneer factors like FOXA1 open chromatin to control gene activity. New tools show FOXA1’s precise role in cancer, revealing it can activate or repress genes critical for tumor growth.

    More Related Videos

    The Power of Simplicity: Sea Urchin Embryos as in Vivo Developmental Models for Studying Complex Cell-to-cell Signaling Network Interactions
    07:34

    The Power of Simplicity: Sea Urchin Embryos as in Vivo Developmental Models for Studying Complex Cell-to-cell Signaling Network Interactions

    Published on: February 16, 2017

    8.4K
    Adenovirus-mediated Genetic Removal of Signaling Molecules in Cultured Primary Mouse Embryonic Fibroblasts
    11:00

    Adenovirus-mediated Genetic Removal of Signaling Molecules in Cultured Primary Mouse Embryonic Fibroblasts

    Published on: September 9, 2010

    25.8K

    Related Experiment Videos

    Last Updated: Mar 12, 2026

    Dissecting Cell-Autonomous Function of Fragile X Mental Retardation Protein in an Auditory Circuit by In Ovo Electroporation
    11:10

    Dissecting Cell-Autonomous Function of Fragile X Mental Retardation Protein in an Auditory Circuit by In Ovo Electroporation

    Published on: July 6, 2022

    2.7K
    The Power of Simplicity: Sea Urchin Embryos as in Vivo Developmental Models for Studying Complex Cell-to-cell Signaling Network Interactions
    07:34

    The Power of Simplicity: Sea Urchin Embryos as in Vivo Developmental Models for Studying Complex Cell-to-cell Signaling Network Interactions

    Published on: February 16, 2017

    8.4K
    Adenovirus-mediated Genetic Removal of Signaling Molecules in Cultured Primary Mouse Embryonic Fibroblasts
    11:00

    Adenovirus-mediated Genetic Removal of Signaling Molecules in Cultured Primary Mouse Embryonic Fibroblasts

    Published on: September 9, 2010

    25.8K

    Area of Science:

    • Molecular Biology
    • Cancer Biology
    • Epigenetics

    Background:

    • Pioneer factors, such as FOXA1, are crucial for controlling gene transcription by modifying chromatin accessibility.
    • Studying pioneer factor activity with kinetic precision has been limited by a lack of suitable chemical tools.
    • FOXA1 is a key factor in the progression of breast and prostate cancers.

    Purpose of the Study:

    • To develop novel chemical tools for precise kinetic studies of FOXA1 activity.
    • To investigate the precise role of FOXA1 in chromatin remodeling and gene transcription.
    • To understand how FOXA1's pioneering activity influences cancer-relevant transcriptional programs.

    Main Methods:

    • Development of a dTAG-based system for small molecule-induced degradation of FOXA1.
    • Coupling pharmacological perturbations with rapid measurements of chromatin structure and function.
    • Analysis of genomic localization, chromatin accessibility, and gene expression changes following FOXA1 modulation.

    Main Results:

    • FOXA1 exclusively initiates chromatin opening at its specific genomic binding sites.
    • This chromatin opening by FOXA1 can lead to both gene activation and repression, depending on the surrounding chromatin environment.
    • These regulatory effects were observed for androgen receptor (AR) target genes and other cancer-associated genes.

    Conclusions:

    • The developed dTAG system provides a powerful chemical tool for dissecting FOXA1's function with kinetic precision.
    • FOXA1's pioneering activity has a context-dependent effect on gene transcription, leading to both activation and repression.
    • Understanding these regulatory mechanisms is critical for developing targeted therapies for FOXA1-driven cancers.