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μSTRAW: In-Field Detection of Escherichia coli.

Crescenzo Ianniello1,2, Barbara Kasprzyk-Hordern3,4, Nuno M Reis1,2,4,5

  • 1Department of Chemical Engineering, University of Bath, Claverton Down, Bath BA2 7AY, U.K.

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|March 11, 2026
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Summary

A new portable DNA extraction device, μSTRAW, enables rapid detection of Escherichia coli in various settings. This technology significantly reduces reagent use and offers a scalable solution for point-of-care diagnostics.

Keywords:
DNA extractionEscherichia coliLAMPbloodfiltrationmicrofluidicsurine

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Area of Science:

  • Microfluidics
  • Molecular Diagnostics
  • OneHealth Research

Background:

  • Rapid detection of *Escherichia coli* is crucial for public health in diverse OneHealth settings.
  • Conventional microbiology methods for bacteria enumeration are time-consuming (24-48 hours).
  • Existing nucleic acid testing is limited by the lack of scalable, affordable portable DNA extraction methods for point-of-care applications.

Purpose of the Study:

  • To develop a compact, gravity-driven microfluidic device (μSTRAW) for portable DNA extraction.
  • To integrate μSTRAW with a portable Loop-mediated Isothermal Amplification (LAMP) instrument for rapid *E. coli* detection.
  • To assess the efficiency and sensitivity of the μSTRAW-LAMP system in various sample matrices.

Main Methods:

  • Utilized μSTRAW, a microfluidic device with microcapillary strips and magnetic beads (MB) for DNA extraction.
  • Reduced reagent consumption (MB, lysis buffer, proteinase K) compared to standard protocols.
  • Integrated the device with a portable LAMP instrument for simultaneous detection from 8 samples.

Main Results:

  • Achieved a low limit of detection (LLoD) of 1000-5000 CFU/mL in PBS within 120-140 minutes.
  • Improved LLoD to 50-100 CFU/mL with pre-concentration using a syringe filter.
  • Demonstrated effective detection in synthetic urine (100-1000 CFU/mL) and sheep blood (10-23 CFU/mL).

Conclusions:

  • μSTRAW provides a portable, affordable, and scalable solution for DNA extraction.
  • The integrated μSTRAW-LAMP system enables rapid *E. coli* detection at the point-of-care.
  • This technology has transformative potential for OneHealth applications, including environmental monitoring and clinical diagnostics.