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Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing
Published on: July 28, 2017
Comparative plastid genomics of Elaeagnaceae: Resolving phylogeny and revealing structural evolution
Wei Gu1, Ji-Gui Yuan2, Li-Bo Zhao1
1Germplasm Bank of Wild Species, Yunnan Key Laboratory of Crop Wild Relatives Omics, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China; University of Chinese Academy of Sciences, Beijing 101408, China; Key Laboratory of Plant Diversity and Specialty Crops, Chinese Academy of Sciences, Beijing 100093, China.
None:
Despite having an established backbone phylogeny of Elaeagnaceae, the intrageneric relationships within Elaeagnus L. remain elusive, and family-wide plastome evolution is poorly explored. We analyzed 71 plastomes of 62 species, including four newly sequenced Elaeagnus samples, to reconstruct phylogenies using protein-coding genes (PCGs), intergenic spacers (IGS), and complete sequences. Among these datasets, PCGs provided the strongest resolution, clarifying interspecific and infraspecific relationships within Hippophae L. However, Elaeagnus topologies remained poorly resolved across all datasets, consistent with a history of rapid evolutionary radiation. Plastome structure was highly conserved in Elaeagnaceae, except for lineage-specific IR expansion in Hippophae. Additionally, we identified repeat sequences and signals of positive selection. This study provides essential plastomic resources for understanding the phylogenetic complexity and adaptive evolution of Elaeagnaceae.
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