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Updated: Mar 13, 2026

Sequential Extraction of Soluble and Insoluble Alpha-Synuclein from Parkinsonian Brains
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Structure-function relationship of alpha-synuclein fibrillar polymorphs derived from distinct synucleinopathies.

Tetiana Serdiuk1, Virginie Redeker2, Jimmy Savistchenko2

  • 1Institute of Molecular Systems Biology, Department of Biology, ETH Zurich, Zurich, Switzerland.

Molecular Systems Biology
|March 12, 2026
PubMed
Summary

Pathogenic alpha-synuclein (αSyn) structures differ across synucleinopathies like Parkinson's disease (PD), Dementia with Lewy Bodies (DLB), and Multiple System Atrophy (MSA). These structural variations influence protein interactions and cellular responses, offering new therapeutic targets.

Keywords:
Alpha-SynucleinAmyloid StrainsLimited Proteolysis-Coupled to Mass SpectrometryParkinson’s DiseaseStructural Proteomics

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Area of Science:

  • Neuroscience
  • Molecular Biology
  • Biochemistry

Background:

  • Alpha-synuclein (αSyn) aggregation is central to synucleinopathies, including Parkinson's disease (PD), Dementia with Lewy Bodies (DLB), and Multiple System Atrophy (MSA).
  • The distinct pathologies of these diseases are hypothesized to stem from different structural forms (polymorphs) of aggregated αSyn.
  • Understanding these structural differences is crucial for elucidating disease mechanisms and developing targeted therapies.

Purpose of the Study:

  • To investigate the structural variations of pathogenic alpha-synuclein (αSyn) aggregates in distinct synucleinopathies (PD, DLB, MSA).
  • To identify disease-specific protein interactomes and cellular responses associated with different αSyn fibril structures.
  • To explore the role of the ubiquitin-proteasomal system (UPS) in the turnover and degradation of pathogenic αSyn species.

Main Methods:

  • Utilized covalent labeling and limited proteolysis coupled to mass spectrometry (LiP-MS) on αSyn aggregates.
  • Analyzed samples in vitro, within neuronal cells, and directly from patient brain homogenates (PD, DLB, MSA).
  • Employed CRISPR-based tools for genetic modulation of UPS components and assessed their impact on αSyn inclusions.

Main Results:

  • Demonstrated distinct structural differences in pathogenic αSyn from PD, DLB, and MSA.
  • Identified disease-specific ubiquitination patterns, turnover profiles, and fibril interactomes, including components of the UPS.
  • Showed that genetic modulation of specific UPS E3 ligases and VCP reduced αSyn inclusions in a strain-specific manner.
  • Detected disease-specific protein alterations in postmortem brain homogenates correlating with cellular responses to patient-derived fibrils.

Conclusions:

  • Pathogenic αSyn structures are indeed distinct across different synucleinopathies, driving disease-specific cellular processes.
  • The ubiquitin-proteasomal system plays a critical role in αSyn aggregate turnover, with disease-specific interactions influencing degradation resistance.
  • These findings offer a valuable resource for understanding synucleinopathy pathobiology and identifying novel therapeutic targets.