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Recombinant Protein Expression, Crystallization, and Biophysical Studies of a Bacillus-conserved Nucleotide Pyrophosphorylase, BcMazG
Published on: May 16, 2017
Differential Analysis of IAA Anabolism Pathway Based on Bacillus cereus, Bacillus subtilis and Bacillus safensis
Dongyue Hou1, Xuejie Zhai1, Kezhong Zhang1,2
1College of Landscape Architecture, Beijing University of Agriculture, Beijing, China.
Abstract:
Bacteria of the genus Bacillus are widely used in agriculture due to their ability to synthesise IAA, but the metabolic pathways of IAA synthesis and the regulatory mechanisms of key genes remain unclear. In this study, the biochemical characteristics of Bacillus cereus, Bacillus subtilis and Bacillus safensis were detected. It was found that Bacillus subtilis has a higher indoles synthesis efficiency, Bacillus safensis has stronger salt tolerance and Bacillus cereus has more prominent alkali tolerance. Through whole-genome sequencing and comparative analysis, combined with the retrieval of key genes in the tryptophan metabolic pathway and the metabolic analysis of indole compounds, it is clarified that Bacillus cereus has three IAA synthesis pathways, namely TAM, IAM and IPyA; Bacillus subtilis contains IAM and IPyA pathways; and Bacillus safensis only has the IPyA pathway. This study offers new insights into Bacillus metabolism, high-quality strains for microbial agents, and matters for plant-growth bacteria application and agricultural sustainability.
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