[Tissue distribution in rats of Yanlishuang Oral Dripping Pills based on GC-MS and UPLC-MS/MS]

The content levels of borneol, borneol-2-O-glucuronide(B2G), menthol, menthol-1-O-glucuronide(M1G), camphor, and glycyrrhizic acid in various rat tissue were determined after administration of Yanlishuang Oral Dripping Pills, and their distribution characteristics in different tissue were investigated. Normal SD rats were given Yanlishuang Oral Dripping Pills by gavage. Plasma and nine types of tissue(heart, liver, spleen, lung, kidney, intestine, brain, stomach, and pharynx) were collected at different time points after administration. Quantitative analysis methods using gas chromatography-mass spectrometry(GC-MS) and ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) were established to determine the content of the six components in plasma and tissue samples, and to compare the distribution differences among tissue. The results showed that all six prototype components and metabolites were absorbed into the blood and widely distributed in the body, reaching peak levels in plasma and tissue within 30 min and being gradually eliminated at 90 min. The prototype components borneol, menthol, and camphor were mainly distributed in the stomach and intestine; glycyrrhizic acid was mainly distributed in the stomach, intestine, and pharynx, with only small amounts in other tissue. The metabolites B2G and M1G were mainly distributed in the intestine, liver, and kidney. Among them, B2G and glycyrrhizic acid were more abundant in the pharynx. This study revealed the differences in tissue distribution of the prototype components and metabolites of Yanlishuang Oral Dripping Pills in vivo, providing theoretical reference for the study of its pharmacological effects and clinical application.