[Analysis of association between HIF-1α gene polymorphism and different syndromes of early cough variant asthma...

This study aims to explore the association between the polymorphism of HIF-1α gene and the susceptibility to early cough variant asthma(CVA) with Qi deficiency and blood stasis, lung and spleen Qi deficiency, liver fire invading lung, and wind invading lung defense, as well as the lung function. The study selected 120 CVA patients who visited the respiratory department from December 2021 to December 2023 as the research subjects and included them in the case group. According to the classification of TCM syndromes, patients in the case group were divided into Qi deficiency and blood stasis group(n=30), lung and spleen Qi deficiency group(n=26), liver fire invading lung group(n=26), and wind invading lung defense group(n=38). Another 60 healthy individuals who underwent physical examinations during the same period were selected as control group. The clinical data of the case group and the control group were compared. The polymorphism of the HIF-1α gene was analyzed. The distribution of the HIF-1α gene in the case group and the control group was compared. The association between the polymorphism of the HIF-1α gene and the risk of CVA occurrence was analyzed. The polymorphism of the HIF-1α gene in different clinical types of patients was analyzed. The association between the polymorphism of the HIF-1α gene and the clinical types and the association between the polymorphism of the HIF-1α gene and the lung function indicators of patients with Qi deficiency and blood stasis were analyzed. The results showed that compared with those in the control group, the HIF-1α level in the case group was significantly higher, and FVC, FEV_1, and FEV_1/FVC levels were significantly lower(P<0.001). In the additive model and dominant model, the polymorphism of the HIF-1α gene(rs11549465, rs11549467 locus) was statistically significant in the association with the risk of CVA occurrence(P<0.05). The distribution frequency of CT and TT genotypes at the rs11549465 locus, the allele T, the GA and AA genotypes at the rs11549467 locus, and the allele A in the Qi deficiency and blood stasis group, the lung and spleen Qi deficiency group, the liver fire invading lung group, and the wind invading lung defense group was significantly higher than that in the control group(P<0.05). In the additive model and dominant model, the polymorphism of the HIF-1α gene(rs11549465, rs11549467 locus) was statistically significant in the association with the risk of Qi deficiency and blood stasis occurrence(P<0.05). In the additive model and dominant model, the polymorphism of the HIF-1α gene(rs11549465, rs11549467 locus) was statistically significant in the association with the FVC, FEV_1, and FEV_1/FVC of patients with Qi deficiency and blood stasis(P<0.05). The results indicated that the polymorphism of the HIF-1α gene rs11549465 and rs11549467 was closely related to the TCM syndrome classification of early CVA and affected the lung function.