Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Synthetic Biology02:55

Synthetic Biology

5.7K
Synthetic biology is an interdisciplinary science that involves using principles from disciplines such as engineering, molecular biology, cell biology, and systems biology. It involves remodeling existing organisms from nature or constructing completely new synthetic organisms for applications such as protein or enzyme production, bioremediation, value-added macromolecule production, and the addition of desirable traits to crops, to name a few.
Golden rice
Golden rice is a genetically modified...
5.7K
DNA Bacteriophages01:26

DNA Bacteriophages

1.3K
Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...
1.3K
Biosynthesis in Bacteria01:24

Biosynthesis in Bacteria

914
Biosynthesis in bacteria is a fundamental anabolic process that generates essential macromolecules, including proteins, nucleic acids, lipids, and polysaccharides. These macromolecules are critical for cellular growth, replication, and function. The process is tightly regulated and energetically linked to catabolic pathways to ensure optimal resource utilization.Biosynthetic pathways begin with precursor metabolites such as pyruvate, acetyl-CoA, and glucose-6-phosphate derived from glycolysis,...
914
The Central Dogma01:20

The Central Dogma

35.0K
The central dogma explains the flow of genetic information from DNA nucleotides to the amino acid sequence of proteins.
RNA is the Missing Link Between DNA and Proteins
In the early 1900s, scientists discovered that DNA stores all the information needed for cellular functions and that proteins perform most of these functions. However, the mechanisms of converting genetic information into functional proteins remained unknown for many years. Initially, it was believed that a single gene is...
35.0K
Amplifying Signals via Enzymatic Cascade01:22

Amplifying Signals via Enzymatic Cascade

19.0K
When a ligand binds to a cell-surface receptor, the receptor's intracellular domain changes shape, which may either activate its enzyme function or allow its binding to other molecules. The initial signal is amplified by most signal transduction pathways. This means that a single ligand molecule can activate multiple molecules of a downstream target. Proteins that relay a signal are most commonly phosphorylated at one or more sites, activating or inactivating the protein. Kinases catalyze...
19.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Super-Resolution Ultrasound Based Cell Tracking With Polymeric Nanobubbles.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

The mechanochemical activation of a pyrimidine dimer.

Chemical science·2026
Same author

Two-Dimensional Polymers as Modular Metal-Free Solid-State Catalysts for Efficient Sono-Piezo-Photocatalytic Hydrogen Peroxide Production.

Angewandte Chemie (International ed. in English)·2026
Same author

Coupling Electrochemical NO Reduction with Selective Catalytic Reduction for Off-Gas Treatment Without External Reducing Agents.

Industrial & engineering chemistry research·2026
Same author

Precise Mechanochemical Scission of DNA Guided by Secondary Structures.

Journal of the American Chemical Society·2026
Same author

Stimuli-responsive microgels cross-linked through supramolecular interactions of peptides.

Journal of colloid and interface science·2026
Same journal

Efficient Syngas Photoproduction Enabled by Electronic Engineering of Co-Immobilized Imine COFs.

Angewandte Chemie (International ed. in English)·2026
Same journal

Pathway Controlled Phase Separation of Minimal Building Blocks Utilizing a Dissociative Chemical Transformation.

Angewandte Chemie (International ed. in English)·2026
Same journal

Interaction Hierarchy and Polymorphic Structure-Property Dynamics in Luminescent Molecular Crystals.

Angewandte Chemie (International ed. in English)·2026
Same journal

The Role of Zn-Hf Site Proximity and Oxygen Vacancies for Methanol Formation Over ZnHfO<sub>x</sub> Catalysts Under CO<sub>2</sub> Hydrogenation Conditions.

Angewandte Chemie (International ed. in English)·2026
Same journal

Breaking the Linear Scaling Relationship: Bioinspired Electronic Coupling in S-Bridged Fe-Fe Dual Sites for Efficient Oxygen Reduction.

Angewandte Chemie (International ed. in English)·2026
Same journal

Programming Bio-Bio Electronic Interfaces for Light-Driven Interspecies Electron Transfer.

Angewandte Chemie (International ed. in English)·2026
See all related articles

Related Experiment Video

Updated: Mar 13, 2026

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks
07:50

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks

Published on: November 25, 2015

15.0K

Dissipatively Fueled Unidirectionally Communicating DNA Circuits That Control Biocatalysis.

Philippe Jung1,2, Daniel Felder2,3, Gurudas Chakraborty2

  • 1Institute of Technical and Macromolecular Chemistry, RWTH Aachen University, Aachen, Germany.

Angewandte Chemie (International Ed. in English)
|March 12, 2026
PubMed
Summary
This summary is machine-generated.

Researchers developed out-of-equilibrium DNA circuits that control enzyme activity. These systems mimic life

Keywords:
DNA networksbiocatalysisdissipative self‐assemblyunidirectional communication

More Related Videos

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
09:26

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

4.9K
Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins
10:46

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Published on: October 18, 2022

2.3K

Related Experiment Videos

Last Updated: Mar 13, 2026

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks
07:50

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks

Published on: November 25, 2015

15.0K
DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
09:26

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

4.9K
Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins
10:46

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Published on: October 18, 2022

2.3K

Area of Science:

  • Biochemistry and Molecular Biology
  • Synthetic Biology
  • Chemical Engineering

Background:

  • Living systems exhibit dynamic, self-reorganizing properties driven by energy consumption and spatiotemporal biocatalysis.
  • Synthetic systems often lack the dynamic and adaptive control seen in biological processes.
  • Controlling enzyme activity in synthetic systems remains a challenge.

Purpose of the Study:

  • To design unidirectionally communicating, out-of-equilibrium DNA circuits for network-guided control of enzyme biocatalytic activity.
  • To mimic life's ability to dynamically regulate processes through energy consumption and controlled biocatalysis.
  • To establish a framework for temporal regulation of enzymes using fuel-driven dissipation.

Main Methods:

  • Development of DNA circuits utilizing programmed, dissipative manipulation for information transfer.
  • Transient activation of a DNAzyme to generate fuel for temporal activation of trypsin.
  • Employing fuel-driven dissipation to regulate nucleic acid and protein-based enzymes in cyclic processes.
  • Utilizing rapid DNA strand hybridization to attain transient states and exonuclease digestion to regenerate equilibrium.
  • Experimental and computational methodologies for system analysis and control.

Main Results:

  • Demonstrated unidirectionally communicating DNA circuits that enable network-guided control of enzyme activity.
  • Achieved temporal regulation of enzyme activity through fuel-driven dissipation and cyclic processes.
  • Precisely controlled the lifetime of transient states by manipulating fuel and exonuclease concentrations and DNA strand kinetics.

Conclusions:

  • Successfully created out-of-equilibrium DNA circuits capable of spatiotemporally controlling biocatalytic processes.
  • Established a novel information-transfer framework for regulating enzyme activity, inspired by life's dynamic nature.
  • The developed system offers precise control over enzyme function, paving the way for advanced synthetic biological applications.