Screening and Identification of Triglyceride-Lowering Lactic Acid Bacteria and Preparation of Probiotic Agents

To isolate lactic acid bacteria with significant triglyceride-degrading ability from traditional fermented foods in Guizhou and develop probiotic tablets, providing a basis for the development of functional probiotics. Strains with triglyceride-degrading ability were screened from 26 isolated strains. Morphological analysis, 16S rRNA gene sequencing, and stress resistance tests were conducted. The lyoprotectant composition for tablet preparation was optimized, and the stability and survival rate of the probiotic tablets were evaluated. Six LAB strains were selected, all exhibiting a triglyceride degradation rate exceeding 60%. Among them, PYC02 (Limosilactobacillus fermentum), SZ02 (Weissella cibaria), and PLHB02 (Weissella cibaria) showed degradation rates greater than 80%. PYC02 and PLHB02 maintained survival rates above 80% under conditions of pH 3 and 0.3% bile salts. The optimal lyoprotectant composition for maximum probiotic survival was 8% defatted milk powder, 5% sodium alginate, 2% maltodextrin, and 8% trehalose. After 30 days of storage at 4°C and 25°C, the survival rates of the probiotic tablets exceeded 80% and 60%, respectively, with triglyceride degradation rates greater than 60%. The triglyceride-degrading effects of PYC02 and PLHB02 were significant. These strains exhibited strong stress resistance and high safety, showing potential for the development of functional probiotics, offering new approaches for the prevention and treatment of hypertriglyceridemia.