Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Marine sulfated polysaccharides as biofunctional agents for enhancing hemocompatibility and endothelialization of tissue-engineered vascular grafts.

Materials horizons·2026
Same author

Aromatic Copolyesters Based on Poly(butylene furanoate) and Poly(butylene isophthalate) for Small-Diameter Vascular Applications.

ACS biomaterials science & engineering·2026
Same author

Dual-Responsive Hydrogels Engineer Anisotropic Cellular Microenvironment to Modulate Stem Cell Organization and Fate.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

Chondrogenic differentiation of human periosteum-derived cells in spheroids, HAMA hydrogels, and bioprinted constructs: comparison of kartogenin and TGF-<i>β</i>1.

Biomedical materials (Bristol, England)·2026
Same author

Copolymers of Poly(Butylene Trans-1,4-Cyclohexanedicarboxylate)/Pripol as New Biomaterial Platform for Small Diameter Vascular Graft.

Advanced healthcare materials·2026
Same author

High-enthalpy Larderello geothermal system, Italy, powered by thousands of cubic kilometres of mid-crustal magma.

Communications earth & environment·2026

Related Experiment Video

Updated: Mar 13, 2026

Fabrication of Engineered Vascular Flaps Using 3D Printing Technologies
08:31

Fabrication of Engineered Vascular Flaps Using 3D Printing Technologies

Published on: May 19, 2022

4.7K

Bioinspired scaffold design using a custom Voronoi path generator for extrusion-based 3D printing.

Federico Farina1,2,3, Michela Licciardello1,2, Lorenzo Moroni3

  • 1Politecnico di Torino, Department of Mechanical and Aerospace Engineering, Torino, 10129, Italy. chiara.tondaturo@polito.it.

Biomaterials Science
|March 12, 2026
PubMed
Summary

Researchers created a biomimetic Voronoi pattern using 3D printing and electrospinning to build better in vitro tissue models, specifically mimicking the lung's alveolar-capillary barrier for cell culture applications.

More Related Videos

Core/shell Printing Scaffolds For Tissue Engineering Of Tubular Structures
05:52

Core/shell Printing Scaffolds For Tissue Engineering Of Tubular Structures

Published on: September 27, 2019

10.0K
Printing Thermoresponsive Reverse Molds for the Creation of Patterned Two-component Hydrogels for 3D Cell Culture
10:49

Printing Thermoresponsive Reverse Molds for the Creation of Patterned Two-component Hydrogels for 3D Cell Culture

Published on: July 10, 2013

15.7K

Related Experiment Videos

Last Updated: Mar 13, 2026

Fabrication of Engineered Vascular Flaps Using 3D Printing Technologies
08:31

Fabrication of Engineered Vascular Flaps Using 3D Printing Technologies

Published on: May 19, 2022

4.7K
Core/shell Printing Scaffolds For Tissue Engineering Of Tubular Structures
05:52

Core/shell Printing Scaffolds For Tissue Engineering Of Tubular Structures

Published on: September 27, 2019

10.0K
Printing Thermoresponsive Reverse Molds for the Creation of Patterned Two-component Hydrogels for 3D Cell Culture
10:49

Printing Thermoresponsive Reverse Molds for the Creation of Patterned Two-component Hydrogels for 3D Cell Culture

Published on: July 10, 2013

15.7K

Area of Science:

  • Biomaterials Engineering
  • Tissue Engineering
  • Biotechnology

Background:

  • Natural biological systems exhibit complex, specialized patterns crucial for tissue and organ function.
  • Engineered microenvironments require accurate biomimicry to support cell cultures and develop relevant biological models.
  • Recreating physiological arrangements is key for advancing tissue engineering and in vitro studies.

Purpose of the Study:

  • To develop a novel method for fabricating biomimetic tissue constructs using additive manufacturing.
  • To create an in vitro model of the alveolar-capillary barrier by mimicking lung tissue organization.
  • To integrate digital design with hybrid fabrication techniques for advanced tissue engineering.

Main Methods:

  • Designed a Python-based software tool to generate Voronoi patterns.
  • Utilized melt electrowriting (MEW) and fused deposition modelling (FDM) for extrusion-based additive manufacturing.
  • Integrated printed Voronoi structures with electrospun nanofibrous membranes to create a multiscale construct.

Main Results:

  • Successfully fabricated a multiscale construct combining additive manufacturing fidelity with electrospinning's ECM-like features.
  • Cultured alveolar epithelial and endothelial cells on the construct to mimic the alveolar-capillary barrier in vitro.
  • Achieved complete cell coverage and physiological-like organization under air-liquid-interface (ALI) conditions for 10 days.

Conclusions:

  • Introduced a flexible, hybrid approach merging digital design and fabrication for in vitro tissue models.
  • Demonstrated the potential of Voronoi patterns and hybrid fabrication for mimicking complex physiological environments.
  • Developed a promising platform for creating advanced in vitro tissue models that closely resemble native tissue architecture and function.