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Sampling Sub-Diffraction Temperature Gradients with Spectrally Orthogonal Nanoparticle Luminescence.

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Updated: Mar 14, 2026

Studying the Effects of Temperature on the Nucleation and Growth of Nanoparticles by Liquid-Cell Transmission Electron Microscopy
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Upconverting Nanoparticle Thermometry beyond the Diffraction Limit.

Benjamin Harrington1, Ziyang Ye1,2, Laura Signor3

  • 1Materials Science Program, University of Rochester, Rochester, New York 14627, United States.

Accounts of Chemical Research
|March 12, 2026
PubMed
Summary
This summary is machine-generated.

Researchers developed new methods for nanoscale temperature measurement using upconverting nanoparticles (UCNPs) that overcome the diffraction limit. These techniques enable precise temperature mapping in microelectronics and biological systems.

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Area of Science:

  • Nanotechnology
  • Materials Science
  • Optical Physics

Background:

  • Nanoscale temperature measurement is crucial for microelectronics, batteries, and biological studies.
  • Upconverting nanoparticles (UCNPs) offer advantages for luminescence thermometry due to their stability and tunable properties.
  • Conventional optical thermometry is limited by diffraction, hindering nanoscale resolution.

Purpose of the Study:

  • To develop strategies for surpassing the diffraction limit in upconverting nanoparticle (UCNP) thermometry.
  • To enable precise temperature measurements at the nanoscale for various applications.
  • To explore advanced optical techniques for enhanced spatial resolution in thermometry.

Main Methods:

  • Investigating single UCNP thermometry with subdiffraction resolution, considering factors like UCNP size and optical environment.
  • Employing spectrally orthogonal temperature-dependent luminescence from different UCNP compositions to map multiple temperature points.
  • Adapting stimulated emission depletion (STED) super-resolution imaging for STED nanothermometry.

Main Results:

  • Achieved subdiffraction limited spatial resolution in UCNP thermometry, governed by UCNP size.
  • Resolved temperature differences over sub-110 nm distances using UCNPs with spectrally orthogonal luminescence.
  • Demonstrated STED nanothermometry to reveal temperature gradients undetectable by diffraction-limited methods.

Conclusions:

  • Developed and demonstrated three key strategies for UCNP thermometry beyond the diffraction limit.
  • These advanced techniques offer significant improvements in spatial resolution for nanoscale temperature measurements.
  • Future research needs include enhancing measurement capabilities and promoting broader adoption of these emerging techniques.